Iron is an essential element for living organisms that plays critical roles in the process of bacterial growth and metabolism. However, it remains to be elucidated whether piuB encoding iron-uptake factor is involved in iron uptake and pathogenicity of Xanthomonas axonopodis pv. glycines (Xag). To investigate the function of piuB, we firstly generated a piuB deletion mutant (ΔpiuB) by homologous recombination. Compared with the wild-type, the piuB mutant exhibited significantly reduced growth and virulence in host soybean. The mutant displayed markedly increased siderophore secretory volume, and its sensitivity to Fe³⁺, Cu²⁺, Zn²⁺ and Mn²⁺ was significantly enhanced. Additionally, the H₂O₂ resistance, exopolysaccharide yield, biofilm formation, and cell mobility of ΔpiuB were significantly diminished compared to that of the wild-type. The addition of exogenous Fe³⁺ cannot effectively restore the above characteristics of ΔpiuB. However, expressing piuB in trans rescued the properties lost by ΔpiuB to the levels in the wild-type. Taken together, our results demonstrated that PiuB is a potential factor for Xag to assimilate Fe³⁺, and is necessary for Xag to be pathogenic in host soybean.
Iron ; Glycine max ; Virulence ; Xanthomonas axonopodis/genetics* ; Hydrogen Peroxide

Objective To evaluate the molluscicidal effect of spraying different formulations of niclosamide ethanolamine salt with drones against Oncomelania hupensis in ditches. Methods A semi-dry and semi-wet ditch with O. hupensis snails was selected in the second branch field of Jiangbei Farm, Jiangling County, Hubei Province in May 2023, and divided into 4 experimental areas, named groups A1, A2, B1 and B2. Environmental cleaning was performed in groups A1 and B2, and was not conducted in groups A2 or B2. Then, 50% wettable powder of niclosamide ethanolamine salt was sprayed with drones at an effective dose of 2 g/m² in groups A1 and A2, and 5% niclosamide ethanolamine salt granule was sprayed with drones at an effective dose of 2 g/m² in groups B1 and B2. O. hupensis snails were surveyed using the systematic sampling method 1, 3, 5, 7, 14 days after spraying, and the natural mortality and corrected mortality of O. hupensis snails were calculated. Results The occurrence of frames with living snails, mean density of living snails and natural mortality of snails were 97.50% (117/120), 6.30 snails/0.1 m² and 1.18% (9/765) in the test ditch before spraying, respectively. There were significant differences in the mortality of snails among four groups 1, 3, 5, 7 and 14 days after spraying niclosamide formulations with drones (χ² = 17.230, 51.707, 65.184, 204.050 and 34.435, all P values < 0.01). The overall mortality rates of snails were 94.51% (1 051/1 112), 79.44% (908/1 143), 96.54% (977/1 012) and 88.55% (1 021/1 153) in groups A1, A2, B1 and B2 (χ² = 207.773, P < 0.05), respectively. In addition, there was no significant difference in the overall snail mortality between groups A1 and B1 (P > 0.05), and the snail mortality in groups A1 and B1 were both statistically different from that in groups A2 and B2 (all P values < 0.05). Conclusion Both 50% wettlable powder of niclosamide ethanolamine salt and 5% niclosamide ethanolamine salt granule sprayed with drones are active against O. hupensis snails in ditches, and environmental cleaning may improve the molluscicidal effect.

Urinothorax is an uncommon complication following percutaneous nephrolithotomy. We present a case of dyspnea and fever after a percutaneous nephrostolithotomy, next chest CT indicated a significant left pleural effusion and urinothorax was confirmed by the pleural fluid analysis.Chest tube placement did not improve the patient’s dyspnea. The patient finally recovered after the thoracoscopic surgery.

Urosepsis caused by upper urinary tract stone obstruction is a common critically disease in urology.However, it rarely occurs in the patient who underwent a dialysis with uremia.We report a patient who underwent an implantation of ureteral stent to control the infection, and we saved the patient with perinephric hematoma following the surgery. We removed the stones in the left ureteral through a flexible ureteroscope two month later.The hematoma was completely absorbed 6 months after the implantation of ureteral stent.

Objective:To evaluate the teaching effect of online clinical nursing practice in Psychiatry Department for undergraduate nursing students by using Web standardized patients (Web-SP) .Methods:In June 2021, 219 undergraduate nursing students from a university in Beijing were selected as research subjects by convenient sampling. We organized a week of online nursing practice in Psychiatry Department, set up an online practice teaching and research group, recruited and trained 15 standardized patients (SP) among hospital nurses, and designed 12 roles including schizophrenia patients, depression patients, and bipolar disorder patients with typical symptoms according to the actual clinical cases. According to the practice plan, an online practice class was opened on Tencent conference platform. The clinical teachers broadcastedand interviewed SP and SP's family members on the spot. Studentsinteracted with the online teachers and SP through video, voice or text. After the practice, 219 undergraduate nursing students were issued SP Simulated Quality of Standardized Patient Scale (SQSPS) and self-made Web-SP Use Effect Evaluation Questionnaire in Psychiatry Department online. A total of 219 questionnaires were distributed online, and 219 valid questionnaires were recovered, with a valid recovery rate of 100%.Results:The total score of SQSPS of 219 nursing students was (92.08±10.89) . The overall evaluation score of nursing students on the application of Web-SP in online practice was (46.52±4.60) , and the highest score was "deepened empathy for patients", which was (4.79±0.47) . The second was "deepened the understanding of psychiatric nursing", which was (4.75±0.68) . The third was "understanding the impact of symptoms on patients", which was (4.73±0.52) .Conclusions:Applying Web-SP to online clinical nursing practice in Psychiatry Department can meet the practice needs of nursing students for Psychiatric Department and nursing courses, and Web-SP after systematic training can replace real patients to achieve teaching goals.

【Objective】 To investigate the current situation concerning volume control of red blood cells in additive solution produced by blood service in Chongqing, and to lay a foundation for promoting the homogenization of preparation process of red blood cells in additive solution. 【Methods】 A questionnaire was designed to investigate the factors related to the preparation of red blood cells in additive solution. The questionnaire was sent by Chongqing Association of Blood Transfusion via E-mail to 18 blood services in the city, and the collected data was sorted, revised and analyzed by research team. 【Results】 A total of 18 blood services(including 1 blood center + 1 sub-center, 6 central blood stations and 11 central blood banks) returned the questionnaires. The results showed that there were differences among blood services across Chongqing, regarding the centrifugal parameters during preparation, the operation mode and monitoring situation of the capacity control during preparation, and the formulation of the capacity standard of red blood cells in additive solution etc. 【Conclusion】 The preparation process of red blood cells in additive solution, produced by Chongqing blood services, should be further standardized, and the capacity control method of this product in Chongqing should be gradually unified to achieve regional homogeneity and to ensure blood safety.

Objective To analyze the spatial clustering of human schistosomiasis at the village level in key counties in Hubei Province, to provide scientific evidence for formulating strategies for human schistosomiasis prevention and control in the next stage. Methods Gong^'an County and Jiangling County in Hubei Province were selected as representative counties for this study. A town or village was set up as a research unit. Schistosomiasis cases of a positive fecal examination in 2015 and cases with positive detection for schistosomiasis serological antibody titer equal or above 80 in 2016-2018 were selected as research subjects in these two counties. The Kulldorff circular scan statistic was used for the spatial clustering analysis of human schistosomiasis infection status in the population. Results There was spatial clustering of positive schistosomiasis cases of fecal examination, at the level of a town or village in both counties in 2015. There was spatial clustering of positive human serological antibody detection at the level of town or village from 2016-2018. Eighty-six endemic villages in five towns in the northeast of Gong^'an County, along the Yangtze river,including Mahaokou Town, Zhakou Town, Yangjiachang Town, Jiazhuyuan Town and Douhuti Town, were the most prominent. There was no spatial clustering of positive results of human serological antibody detection at the town and village level in Jiangling County, in2017, while there was spatial clustering of human serological antibody detection in 2016 and 2018,respectively. Fifty-seven endemic villages in two towns (Puji Town and Xionghe Town) in the southwest of Jiangling County, along the Yangtze river,were the most prominent. Conclusion There were spatial clustering of human schistosomiasis epidemic at village level both in Gong^'an County and Jiangling County, Hubei Province. Compared with the previous studies, there was a trend of shrink and decline of clustering areas. Therefore, the current situation of the epidemic has put forward higher requirements for the implementation of precise prevention and control in the progress of schistosomiasis elimination work in various epidemic areas.

Objective:To explore the current status of protective restraint during hospitalization in patients with affective disorders.Methods:A self-made questionnaire was used to investigate 400 patients with convalescent affective disorder in a psychiatric hospital in Beijing from November 2018 to April 2019.Results:Among 400 patients with affective disorders, 158 cases had restraint experience, and the incidence of protective restraint was 39.50%. Among the physical influences caused by protective restraint, 147 cases (93.03%) of limb movement were restricted, 133 cases (84.17%) were unable to defecate on their own, and 88 cases (55.69%) had limb pain. Among the psychological influences caused by protective restraint, 83 cases (52.53%) were helpless, 82 cases (51.89%) were aggrieved, 78 cases (49.37%) were powerless and 68 cases (43.04%) were fearful. There were statistically significant differences in age, gender, marital status, number of hospitalizations, and hospitalization diagnosis among patients with protective restraint experience, witnessed protective restraint and never exposed to protective restraint ( P<0.05) . Conclusions:Patients with affective disorders have a high rate of protective restraint. The physical influence and negative psychological experience of patients during and after the protective restraint is a potential threat to the establishment of the doctor-patient treatment alliance. Health education of protective restraint knowledge should be strengthened for inpatients, and comprehensive health education, nursing measures, and psychological interventions should be provided for protective restraint patients, so as to improve the misunderstanding of protective restraint in patients with affective disorders and reduce the negative experience and psychological influences of protective restraint in patients.

Objective To analyze the TCM literature of stomachache based on content analysis, and to explore the law of selecting points in nursing of qi stagnation syndrome. Methods Terms such as"stomachache","distension","belching","gastritis""functional dyspepsia"and"peptic ulcer"were used as the characteristic words, and the CNKI, Wanfang data, VIP, CBM, Pubmed and Embase databases were retrieved to find literature of acupoint therapies for this symptom, while snowball was conducted to expand the literature. SPSS20.0 and Rost Content Mining 5.8 softwares were used to analyze the contents of the included literature, and to explore the syndrome differentiation rules of acupoint therapy in patients with stomachache of qi stagnation. Results Totally 279 Chinese and 27 English articles were included. Through bibliometric analysis, word frequency statistics and cluster analysis, the most commonly used acupoint therapies included acupuncture, acupoint massage, acupoint application, acupoint embedding, acupoint injection. The most common selected acupoints of qi stagnation syndrome was Zusanli, Zhongwan, Neiguan, Taichong, Qimen, Zhangmen, Pishu, Weishu and Ganshu. Conclusions The application of content analysis method in the field of TCM nursing provides a scientific method for summarizing the literature in the future, and is worthy of popularization and application in clinical nursing.

Objective To evaluate the effectiveness of swallowing training supplemented with neuromuscular electrical stimulation to provide a reference for clinical treatment and further study.Methods Reports of randomized and controlled trials of surface neuromuscular electrical stimulation in treating post-stroke dysphagia were sought in the Cochrane library,the PubMed and Embase databases,the Cumulative Index to Nursing and Allied Health Literature (CINAHL),and also in the ProQuest,PsycARTICLES,CBMdisc,China National Knowledge Infrastructure (CNKI),CQVIP database and Wanfang databases.All of the literature found was evaluated by 2 researchers according to predefined inclusion and exclusion criteria and the data were extracted and combined.Then meta-analysis was performed using version 5.3 of the RevMan software package.Results Eleven randomized and controlled trials involving 576 patients were included in the meta-analysis.Together,the data showed that swallowing training supplemented by neuromuscular electrical stimulation is significantly more effective than swallowing training alone in improving swallowing function.It reduces the risk of aspiration and improves quality of life.It does not,however,generally shorten the pharyngeal transmit time.Conclusions Swallowing training supplemented with neuromuscular electrical stimulation is a promising approach for treatment of post-stroke dysphagia and warrants further study.