Objective To observe the effect of Shenfu injection on lung injury caused by hemor-rhagic shock(HS)in rats and explore the related potential mechanism.Methods Thirty-six SPF healthy male SD rats,aged 16-17 weeks,weighing 400-600 g,were randomly divided into three groups:sham op-eration group(group SH),HS group(group HS),and Shenfu injection group(group SF),12 rats in each group.In group SH,only the right femoral vein and femoral artery were separated after anesthesia,and ve-nous catheterization was not performed.HS model was established in groups SF and HS.In group HS,liquid resuscitation was performed through an intravenous catheter,and the resuscitation fluid consisted of the auto-blood lost and the compound sodium chloride injection equivalent to 1.5 times the blood loss and 10 ml/kg normal saline.In group SF,the resuscitation fluid consisted of the lost autoblood and the compound sodium chloride injection equivalent to 1.5 times the blood loss and Shenfu injection 10 ml/kg.The whole perfusion time was about 60 minutes.Six rats in the three groups were randomly anesthetized 24 and 48 hours after op-eration.The wet/dry weight ratio(W/D)of lung tissues was detected.The concentrations of interleukin-6(IL-6),IL-17,IL-10,and transforming growth factor-β(TGF-β)were detected by ELISA,the mRNA ex-pression of retinoic acid-related orphan nuclear receptor γt(RORγt),transcription factor forkhead box pro-tein 3(Foxp3),and hypoxia-inducible factor-1α(HIF-1α)in lung tissues were detected by PCR.The pro-tein contents of RORγt,Foxp3,HIF-1α,aquaporin 1(AQP1),and AQP5 in lung tissue were detected by Western blot.Pathological changesunder HE staining light microscope and lung injury scores were observed.Results Compared with 24 hours after operation,W/D,the concentrations of IL-6 and IL-17,mRNA ex-pression and protein content of RORγt and HIF-1α,and lung injury score were significantly decreased(P＜0.05),the concentrations of IL-10,and TGF-β,Foxp3 mRNA expression and protein content,and AQP1 protein content were significantly increased in group SF 48 hours after operation(P＜0.05).Compared with group SH,W/D,the concentrations of IL-6,IL-17,IL-10,and TGF-β,mRNA expression and protein content of RORγt,Foxp 3,and HIF-1α,and lung injury score were significantly increased(P＜0.05),AQP1 and AQP5 protein contents were significantly decreased in groups HS and SF 24 and 48 hours after operation(P＜0.05),and alveolar structure was damaged under light microscope and alveolar interstitium was filled with a large amount of edematous fluid,during which a large number of inflammatory cells infiltra-ted.Compared with group HS,W/D,the concentrations of IL-6 and IL-17,mRNA expression and protein content of RORγt and HIF-1α,and lung injury score were significantly decreased(P＜0.05),the concen-trations of IL-10 and TGF-β,Foxp3 mRNA expression and protein content,AQP1 and AQP5 protein con-tents were significantly increased in group SF 24 and 48 hours after surgery(P＜0.05),and the alveolar structure was improved under light microscope,and edema was reduced,and the number of inflammatory cells was reduced.Conclusion Shenfu injection can regulate the balance between pro-inflammatory factors IL-6 and IL-17,and anti-inflammatory factors IL-10 and TGF-β,increase the protein content of AQP1 and AQP5 in lung tissue,and decrease the W/D and injury score in lung tissue,thus alleviating lung injury in HS rats.The mechanism may be related to the regulation of HIF-1α-RORγt/Foxp3 balance.

BACKGROUND:Cervical rotatory manipulation is widely used in the treatment of neck-related diseases with a clear curative effect,but it also has some risks in clinical practice.The previous study of our group found that cervical rotatory manipulation can reduce the tensile mechanical properties of the atherosclerotic carotid artery,but it is not clear about the effects of different cervical rotatory manipulations(positioning/non-positioning)and different degrees(mild/moderate/severe)of atherosclerosis on the tensile mechanical properties of the carotid artery. OBJECTIVE:To explore the effects of different cervical rotatory manipulations and different degrees of atherosclerosis on the tensile mechanical properties of the internal carotid artery. METHODS:The 120 male New Zealand rabbits were randomly divided into eight experimental groups with different degrees of atherosclerosis and different cervical rotatory manipulations:severe atherosclerosis + positioning/non-positioning cervical rotatory manipulation,moderate atherosclerosis + positioning/non-positioning cervical rotatory manipulation,mild atherosclerosis + positioning/non-positioning cervical rotatory manipulation,and normal rabbit + positioning/non-positioning cervical rotatory manipulation,as well as three model control groups:mild/moderate/severe atherosclerosis + non-cervical rotatory manipulation,and the blank control group.Two-factor analysis of variance was used to explore the main effects and interactive effects of different cervical rotatory manipulations and different degrees of atherosclerosis on the tensile mechanical properties of the internal carotid artery.One-way analysis of variance was applied to explore the influence of different cervical rotatory manipulations on the tensile mechanical properties of the internal carotid artery under the same degree of atherosclerosis. RESULTS AND CONCLUSION:(1)Both different cervical rotatory manipulations and different degrees of atherosclerosis were the main effect factors affecting the tensile mechanical properties of the internal carotid artery.(2)For both mild and severe atherosclerosis,both positioning and non-positioning cervical rotatory manipulations reduced the maximum stress of the internal carotid artery(P<0.05)and also increased the physiological elastic modulus of the internal carotid artery(P<0.05).(3)For moderate atherosclerosis,positioning and non-positioning cervical rotatory manipulations also increased the physiological elastic modulus of the internal carotid artery(P<0.05).Non-positioning cervical rotatory manipulation reduced the maximum strain of the internal carotid artery(P<0.05),and its maximum strain was also less than the internal carotid artery of the positioning cervical rotatory manipulations(P<0.05).(4)For the normal internal carotid artery,in addition to the maximum strain,both positioning and non-positioning cervical rotatory manipulations had no statistically significant effects on other tensile mechanical indicators of the internal carotid artery(P>0.05).(5)The results suggest that both positioning and non-positioning cervical rotatory manipulations may increase the stiffness of the atherosclerotic internal carotid artery,reducing its elasticity and brittleness.Therefore,both positioning and non-positioning cervical rotatory manipulations may increase the risk of cardiovascular events in mild/moderate/severe atherosclerotic internal carotid artery,and the more severe the atherosclerosis is,the greater the risk of positioning/non-positioning cervical rotatory manipulation treatment,but the risk of positioning cervical rotatory manipulation is not lower than that of non-positioning cervical rotatory manipulation.

Objective To explore the application of drones in blood emergency support during the 19th Asian Games Hangzhou in Shaoxing venue,so as to provide reference for future major events.Methods A retrospective analysis of drone delivery of emergency blood to two designated rescue hospitals in Shaoxing for the Asian Games was conducted.Ten emer-gency and 30 routine land blood delivery records from two designated hospitals in 2023 were randomly selected to compare with the blood delivery using drones.The blood temperature during the drone flight were monitored,the cold chain control during drone flight was evaluated,and the application of drone flight was reviewed.Results During the Hangzhou Asian Games,a total of 14 drones were used for blood delivery.The delivery time(min)by drone from the blood station to one of the designated hospitals was 11.83±0.09,and 20.80±3.29 by emergency land delivery and 23.23±3.54 by routine land de-livery(P＜0.05),meanwhile was 17.50±0.08,24.50±3.27 and 26.57±3.23 respectively to the other hospital(P＜0.05).Compared with land emergency blood delivery and routine blood delivery,blood delivery time by drone was much shorter,demonstrating better timeliness and stability.The temperature monitoring during the drone delivering showed that the suspen-ded red blood cells and platelets were in a range of 3.8-6.8℃ and 21.8-22.8℃,with a temperature difference of 0.5-0.9℃ and 0.2-1.0℃ between takeoff and landing respectively,demonstrating that the blood cold chain was under control,and the delivery ran safely and smoothly.Conclusion During major events,the use of drones for emergency blood delivery ensures the quality of the blood,with good timeliness,feasibility and operability,and is worthy of promotion and applica-tion.

This paper reported the clinical characteristics, diagnosis, and treatment of a case of recurrent endometrial stromal sarcoma with term pregnancy. The patient had undergone laparoscopic surgery to remove hysteromyoma before conception in 2017, which was pathologically diagnosed as low-grade endometrial stromal sarcoma after surgery. Due to her strong reproductive willingness, the patient attempted to conceive in light of her stable condition and no evidence of recurrence and was closely followed up with an informed choice. She conceived successfully in 2020 and underwent regular pregnancy examinations. Ultrasound examination at 37 +4 weeks of gestation revealed a slightly hypoechoic mass of about 6.3 cm×4.5 cm size in the pelvic cavity. After admission, a pelvic MRI indicated multiple solid nodules in the right adnexa uteri and beside the iliac vessels in the left pelvic wall and anterior pelvic wall with the larger one being about 58 mm×28 mm. Diffusion-weighted imaging showed multiple pelvic nodules and masses with significant diffusion restriction. The patient was diagnosed as having multiple solid nodules and masses in the pelvic cavity, and the recurrence of sarcoma was highly suspected. Brain CT and lung CT showed no obvious metastatic lesions. A consultation involving the Department of Gynecological Tumor Chemoradiotherapy was held and the sarcoma recurrence during pregnancy was prenatally diagnosed. After ruling out the contraindications for surgery, a cesarean section was performed in the lower segment of uterus under general anesthesia and a live female baby was delivered at 38 ＋3 weeks. The excised mass was confirmed as recurrent uterine stromal sarcoma by rapid freezing pathology during cesarean section. A combination surgery was performed subsequently, including total extra-fascial hysterectomy, bilateral oophorectomy, bilateral salpingectomy, appendectomy, greater omentum resection, pelvic lesion resection (right side), and pelvic adhesiolysis. Recurrent low-grade uterine stromal sarcoma was reconfirmed by postoperative pathology. The patient was discharged after recovery. After two years of follow-up, no distant metastasis recurrences were found.

Objective To investigate the nutritional status and dietary structure of tuberculosis patients among dif-ferent populations, analyze the factors influencing the nutritional status of tuberculosis patients, and provide theo-retical basis for improving clinical nutrition and related issues in tuberculosis patients.Methods Tuberculosis pa-tients, non-tuberculosis patients, and healthy individuals were randomly selected for a questionnaire survey.De-scriptive analysis was conducted using SPSS 20.0 software.Statistical description was performed using rates and composition ratios, and qualitative data were described using relative numbers.Chi-square test was used to compare overall rates and composition ratios among different health conditions groups, with a significance level of α=0.05.Independent factors analysis of nutritional status body mass index (BMI) was conducted using multiple Logistic re-gression analysis for variables with statistically significant differences in the univariate analysis.Results There were differences in the nutritional status (x2 =62.184, P<0.05) and dietary diversity score (x2 =64.049, P<0.05) among tuberculosis patients, non-tuberculosis patients, and healthy individuals.Univariate analysis of nutri-tional status BMI showed statistically significant differences in gender, smoking, meat-based diet, vegetable-based diet, moderate diet diversity score, and 6 other variables for tuberculosis patients (P <0.05) , and in gender, age, ethnicity, marital status, occupation, education level, smoking, drinking white wine, drinking beer, meat-based diet, moderate diet, and 11 other variables for healthy individuals (P<0.05) .The variables with statisti-cally significant differences in the univariate analysis were included in the multiple ordinal logistic regression analy-sis model for both tuberculosis patients and healthy individuals.The results showed that the level of education, veg-etable intake, moderate food diversity score (DDS) of 4-6 were independent influencing factors of nutritional sta-tus BMI among tuberculosis patients (P<0.05);marital status was an independent influencing factor of nutritional status BMI among non-tuberculosis patients (P<0.05);while gender and occupation were independent influencing factors of nutritional status BMI among healthy individuals (P<0.05).Conclusion The dietary nutritional status of the three population groups varied.Targeted health education should be conducted, especially for tuberculosis patients, to address the issue of uneven dietary intake and promote good dietary habits among local tuberculosis pa-tients.

In April 2024, the World Health Organization/International Agency for Research on Cancer (IARC) published the global cancer statistics 2022 in the CA: Cancer Journal for Clinicians. This report focuses on the incidence and mortality of 36 cancers in 185 countries or territories worldwide, analyzing the differences of gender, geographic region, and the Human Development Index (HDI) level. It is estimated that in the year 2022, there were 19.96 million new cancer cases and 9.74 million cancer deaths worldwide. Lung cancer (2 480 301, 12.4%) was the most frequently diagnosed cancer in 2022, followed by female breast cancer (2 295 686, 11.5%), colorectal cancer (1 926 118, 9.6%), prostate cancer (1 466 680, 7.3%), and gastric cancer (968 350, 4.9%). Lung cancer (1 817 172, 18.7%) was also the leading cause of cancer death, followed by colorectal cancer (903 859, 9.3%), liver cancer (757 948, 7.8%), female breast cancer (665 684, 6.9%), and gastric cancer (659 853, 6.8%). With demographics-based predictions indicating that the number of new cases of cancer will reach over 35 million by 2050. The Beijing Office for Cancer Prevention and Control team has collated this report and briefly interpreted it in combination with the current situation of cancer incidence and mortality in China.

【Objective】 To explore the application of drones in blood emergency support during the 19th Asian Games Hangzhou in Shaoxing venue, so as to provide reference for future major events. 【Methods】 A retrospective analysis of drone delivery of emergency blood to two designated rescue hospitals in Shaoxing for the Asian Games was conducted. Ten emergency and 30 routine land blood delivery records from two designated hospitals in 2023 were randomly selected to compare with the blood delivery using drones. The blood temperature during the drone flight were monitored, the cold chain control during drone flight was evaluated, and the application of drone flight was reviewed. 【Results】 During the Hangzhou Asian Games, a total of 14 drones were used for blood delivery. The delivery time(min) by drone from the blood station to one of the designated hospitals was 11.83±0.09, and 20.80±3.29 by emergency land delivery and 23.23±3.54 by routine land delivery (P<0.05), meanwhile was 17.50±0.08, 24.50±3.27 and 26.57±3.23 respectively to the other hospital(P<0.05). Compared with land emergency blood delivery and routine blood delivery, blood delivery time by drone was much shorter, demonstrating better timeliness and stability. The temperature monitoring during the drone delivering showed that the suspended red blood cells and platelets were in a range of 3.8-6.8℃ and 21.8-22.8℃, with a temperature difference of 0.5-0.9 ℃ and 0.2-1.0℃ between takeoff and landing respectively, demonstrating that the blood cold chain was under control, and the delivery ran safely and smoothly. 【Conclusion】 During major events, the use of drones for emergency blood delivery ensures the quality of the blood, with good timeliness, feasibility and operability, and is worthy of promotion and application.

Objective To explore the application of drones in blood emergency support during the 19th Asian Games Hangzhou in Shaoxing venue,so as to provide reference for future major events.Methods A retrospective analysis of drone delivery of emergency blood to two designated rescue hospitals in Shaoxing for the Asian Games was conducted.Ten emer-gency and 30 routine land blood delivery records from two designated hospitals in 2023 were randomly selected to compare with the blood delivery using drones.The blood temperature during the drone flight were monitored,the cold chain control during drone flight was evaluated,and the application of drone flight was reviewed.Results During the Hangzhou Asian Games,a total of 14 drones were used for blood delivery.The delivery time(min)by drone from the blood station to one of the designated hospitals was 11.83±0.09,and 20.80±3.29 by emergency land delivery and 23.23±3.54 by routine land de-livery(P＜0.05),meanwhile was 17.50±0.08,24.50±3.27 and 26.57±3.23 respectively to the other hospital(P＜0.05).Compared with land emergency blood delivery and routine blood delivery,blood delivery time by drone was much shorter,demonstrating better timeliness and stability.The temperature monitoring during the drone delivering showed that the suspen-ded red blood cells and platelets were in a range of 3.8-6.8℃ and 21.8-22.8℃,with a temperature difference of 0.5-0.9℃ and 0.2-1.0℃ between takeoff and landing respectively,demonstrating that the blood cold chain was under control,and the delivery ran safely and smoothly.Conclusion During major events,the use of drones for emergency blood delivery ensures the quality of the blood,with good timeliness,feasibility and operability,and is worthy of promotion and applica-tion.

Objective To explore the application of drones in blood emergency support during the 19th Asian Games Hangzhou in Shaoxing venue,so as to provide reference for future major events.Methods A retrospective analysis of drone delivery of emergency blood to two designated rescue hospitals in Shaoxing for the Asian Games was conducted.Ten emer-gency and 30 routine land blood delivery records from two designated hospitals in 2023 were randomly selected to compare with the blood delivery using drones.The blood temperature during the drone flight were monitored,the cold chain control during drone flight was evaluated,and the application of drone flight was reviewed.Results During the Hangzhou Asian Games,a total of 14 drones were used for blood delivery.The delivery time(min)by drone from the blood station to one of the designated hospitals was 11.83±0.09,and 20.80±3.29 by emergency land delivery and 23.23±3.54 by routine land de-livery(P＜0.05),meanwhile was 17.50±0.08,24.50±3.27 and 26.57±3.23 respectively to the other hospital(P＜0.05).Compared with land emergency blood delivery and routine blood delivery,blood delivery time by drone was much shorter,demonstrating better timeliness and stability.The temperature monitoring during the drone delivering showed that the suspen-ded red blood cells and platelets were in a range of 3.8-6.8℃ and 21.8-22.8℃,with a temperature difference of 0.5-0.9℃ and 0.2-1.0℃ between takeoff and landing respectively,demonstrating that the blood cold chain was under control,and the delivery ran safely and smoothly.Conclusion During major events,the use of drones for emergency blood delivery ensures the quality of the blood,with good timeliness,feasibility and operability,and is worthy of promotion and applica-tion.

Objective To explore the application of drones in blood emergency support during the 19th Asian Games Hangzhou in Shaoxing venue,so as to provide reference for future major events.Methods A retrospective analysis of drone delivery of emergency blood to two designated rescue hospitals in Shaoxing for the Asian Games was conducted.Ten emer-gency and 30 routine land blood delivery records from two designated hospitals in 2023 were randomly selected to compare with the blood delivery using drones.The blood temperature during the drone flight were monitored,the cold chain control during drone flight was evaluated,and the application of drone flight was reviewed.Results During the Hangzhou Asian Games,a total of 14 drones were used for blood delivery.The delivery time(min)by drone from the blood station to one of the designated hospitals was 11.83±0.09,and 20.80±3.29 by emergency land delivery and 23.23±3.54 by routine land de-livery(P＜0.05),meanwhile was 17.50±0.08,24.50±3.27 and 26.57±3.23 respectively to the other hospital(P＜0.05).Compared with land emergency blood delivery and routine blood delivery,blood delivery time by drone was much shorter,demonstrating better timeliness and stability.The temperature monitoring during the drone delivering showed that the suspen-ded red blood cells and platelets were in a range of 3.8-6.8℃ and 21.8-22.8℃,with a temperature difference of 0.5-0.9℃ and 0.2-1.0℃ between takeoff and landing respectively,demonstrating that the blood cold chain was under control,and the delivery ran safely and smoothly.Conclusion During major events,the use of drones for emergency blood delivery ensures the quality of the blood,with good timeliness,feasibility and operability,and is worthy of promotion and applica-tion.