ObjectiveTo study the dynamic changes of facial nerve F wave in patients with brainstem infarction，and to explore its value in diagnosis and prognosis evaluation of acute brainstem infarction. MethodsFifty-three patients with brainstem infarction were enrolled in our hospital. Electrophysiological examinations were performed on the 3rd，7th，14th，1st and 3rd days of onset，including the occurrence rate and latency of facial nerve F wave，and compared with the healthy side. Three months after onset，the patients were divided into good prognosis group（mRS≤2） and poor prognosis group（mRS>2） according to mRS neurological function score，and 10 healthy adults were included as control group with the same detection method. The incidence and latency of facial nerve F wave at different time points were compared. Results53 patients with brainstem infarction showed a decrease in the occurrence rate of f wave and an extension in the latency period on the 3rd，7th and 14th days of onset respectively，with a statistically significant difference compared with the healthy side（P<0.05）. However，the incubation period recovered at 3 months after onset，with no significant difference compared with the healthy side（P>0.05），while the occurrence rate was still statistically significant compared with the healthy side（P<0.05）. In patients with different types of brainstem infarction，the occurrence rate and incubation period of f wave in each group decreased to different degrees on the 3rd and 7th day，and the difference was not statistically significant（P>0.05）. The occurrence rate and incubation period of f wave in Raymond-Cestan syndrome group first recovered from the 14th day，with significant difference compared with other groups（P<0.05）. The occurrence rate and incubation period of f wave in Millard-Gubber syndrome group were higher than those in other groups at the 1st and 3rd month of onset，the difference was statistically significant（P<0.05）. On the 3rd and 7th day of onset，the incubation period and occurrence rate of f wave in the two groups increased and decreased，with no significant difference（P>0.05）. From the 14th day on，the incubation period and f wave occurrence in the group with good prognosis first recovered in the group with poor prognosis，with significant difference（P<0.05）. At the 3rd month of onset，the occurrence rate of F wave in the group with good prognosis was significantly higher than that in the group with poor prognosis，and the difference was statistically significant. However，the incubation period of the two groups had obvious recovery，and the difference was not statistically significant（P>0.05）. ConclusionThe changes of facial nerve F wave and latency can occur in patients with brainstem infarction in the early stage，but there is no differential diagnosis value for different types of cerebral infarction in the acute stage，which can provide valuable objective basis for prognosis evaluation after the acute stage.

Objective: To evaluate the levels of 1ipoprotein lipase protein (LPL)and mRNA in cerebrospinal fluid (CSF) for children retinoblastoma(RB)and evaluation of the chemotherapy. Methods: Case-control study. Total 36 cases were collected in Beijing Tongren Hospital From October 2015 to May 2017. There were two groups, 19 cases of central nervous system(CNS) metastasis and 17 cases of non CNS metastasis according to laterality, age and gender. The changes of neuronspecific enolase (NSE) in serum and cerebrospinal fluid (CSF), chloride, glucose and quantitative protein and white blood cell count in CSF were compared between the two groups before initiating chemotherapy and after the third and sixth cycles of chemotherapy. LPL expression was assessed by Western blot and RT-PCR.Comparisonsbetweenthetwo groups of general data were performed usingt-test. The measurement data were expressed by mean ± standard deviation, and variance analysis was conducted. Results: The level of CSF-NSE from CNS metastasis group was significantly higher than non CNS metastasis group(F=16.43, P=0.002). The level of serum NSE from CNS metastasis group was significantly higher than non CNS metastasis group before chemotherapy(F=41.06, P=0.006). There were significant differences in the level of serum NSE in CNS metastasis group before and after chemotherapy (F=7.06, P=0.001). CSF-LPL protein expression in the CNS metastasis group was significantly higher than that of non CNS metastasis group (F=2.57, P=0.001). There were significant differences of LPL expression in CNS metastasis group before and after chemotherapy (F=2.63, P=0.003)). Conclusion The expression of LPL protein in CNS may be related to the progression and chemotherapyof RB with CNS metastasis. (Chin J Lab Med, 2018, 41: 608-614)

Objective To evaluate the efficacy and quality of life of segmental bowel resection for bowel endometriosis. Methods Totally 62 symptomatic patients with bowel endometriosis undergoing segmental bowel resection were recruited. A visual analogue scale (VAS) and the 36-item short form health survey (SF-36) questionnaire were administered before and at least 1 year after surgery, respectively. Pregnancy rates were also recorded. Results Sixty-two patients in total underwent follow-up ranging from 12 to 74 months. All patients complained of obvious pain symptoms, including dysmenorrhea, dyspareunia, pain on defecation and chronic pelvic pain. The relief of dysmenorrhea (2.9 ± 2.2 versus 7.5 ± 2.9), dyspareunia (0.7 ± 0.5 versus 4.3 ± 2.2) and pain on defecation (1.6 ± 0.7 versus 7.3 ± 1.9) after surgery was statistically significant (all P<0.01). The scores for all 8 domains of the SF-36 questionnaire were significant improved after segmental bowel resection (all P<0.01). The complication rate was 45% (28/62), including 18 cases of urinary retention, 4 rectovaginal fistulas, 2 cases of vaginal dehiscence, and 1 case each of thrombogenesis, pelvic abscess and general peritonitis. All of the patients with complications recovered well throughout follow-up. The postoperative pregnancy rate of the previous infertile patients was 6/10. Among the 6 gestational cases, 2 had labour, 2 underwent caesarean sections, one had a spontaneous natural abortion, and one underwent uterine curettage. Conclusion Segmental bowel resection could significantly relieve pain and improve quality of life for patients with bowel endometriosis.