Objective:To investigate the efficacy of Balanophora involucrata Hook.f.in treatment of hyperuricemia(HUA)based on network pharmacology,molecular docking,and hyperuricemia models in vivo and in vitro,and to clarify the main targets of its active components and related signaling pathway mechanism.Methods:The potential targets of Balanophora involucrata Hook.f.in treatment of HUA were identified by Databases such as the Traditional Chinese Medicine Database in Taiwan,the Chinese Herbal Medicine Identification Database,Professional Chemical Database,TargetNet Database,SwissTargetPrediction Database,GeneCards,Therapeutic Target Database(TTD),DrugBank Database,DisGeNET Database,Online Mendelian Inheritance in Man(OMIM)Database,and Venny Database.STRING Database and Cytoscape software were used to construct the active component-predictive target network and protein-protein interaction(PPI)network for Balanophora involucrata Hook.f.;topological analysis was used to select the main active components and core targets;Gene Ontology(GO)functional and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway enrichment analysis were performed by R software;AutoDock Vina software was used for molecular docking validation.The NRK-52E cells were divided into blank control group,blank administration group,model group,and different concentrations(2.0,10.0,and 50.0 μmol·L-1)of erythrodiol(EDT)groups.High-performance liquid chromatography culture(HPLC)was used to detect the uric acid(UA)levels in the cell culture supernatants in various groups.The male ICR mice were divided into blank control group,blank administration group,model group,and EDT group;the mice in the last two groups were used to prepare the HUA models;kits were used to detect the levels of UA,creatinine(Cr),and blood urea nitrogen(BUN)in serum of the mice in various groups;the bilateral kidney tissue of the mice was harvested and weighed;the kidney indexes of the mice in various groups were calculated;TUNEL staining was used to observe the apoptosis in kidney tissue of the mice in various groups;Western blotting method was used to detect the expression levels of protein kinase B(AKT),phosphorylated AKT(p-AKT),phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and matrix metalloproteinase-9(MMP-9)proteins in kidney tissue of the mice in various groups.Results:Six active components of Balanophora involucrata Hook.f.were identified,involving 116 intersecting targets and 14 core targets.The enrichment analysis yielded 1 828 GO terms and 145 signaling pathways.The molecular docking results showed that EDT had good binding activity with MMP-9.The high uric acid cell experiment results showed that compared with blank control group,the UA level in the cells in model group was significantly increased(P<0.01);compared with model group,the UA levels in the cells in 2.0,10.0,and 50.0 μmol·L-1 EDT groups were significantly decreased(P<0.01).Compared with blank control group,the levels of UA,Cr,and BUN in serum of the mice in model group were increased(P<0.01),and the kidney indexes were significantly increased(P<0.01);compared with model group,the levels of UA,Cr,and BUN in serum of the mice in EDT group were decreased(P<0.05 or P<0.01),and the kidney index was significantly decreased(P<0.05 or P<0.01).Compared with blank control group,the number of apoptotic cells in kidney tissue of the mice in model group was increased;compared with model group,the number of the apoptotic cells in kidney tissue of the mice in EDT group was significantly decreased.Compared with blank control group,the ratios of p-AKT/AKT and p-PI3K/PI3K and expression level of Bcl-2 protein in kidney tissue of the mice in model group were significantly decreased(P<0.05 or P<0.01),while the expression levels of Bax and MMP-9 proteins were significantly increased(P<0.01);compared with model group,the ratios of p-AKT/AKT and p-PI3K/PI3K and expression level of Bcl-2 protein in kidney tissue of the mice in EDT group were significantly increased(P<0.05 or P<0.01),and the expression levels of Bax and MMP-9 proteins were significantly decreased(P<0.01).Conclusion:The active component of Balanophora involucrata Hook.f.,EDT,has a UA-decreasing effect and may inhibit the apoptosis and alleviate the kidney injury by activating the PI3K/AKT signaling pathway.

Intestinal microbiota is the most complex and important microecosystem in the human body， and gut microbiota dysbiosis is closely associated with the development and progression of acute pancreatitis. Targeted regulation of intestinal microecology in assisting the treatment of acute pancreatitis has attracted more attention in recent years. This article describes the changes in intestinal microbiota and related mechanisms in patients with acute pancreatitis， summarizes the current research status of the use of probiotics， points out the research direction of probiotics as the adjuvant treatment regime， and proposes a new method for predicting the dominant flora in patients with acute pancreatitis， in order to bring new ideas for the treatment of acute pancreatitis.

Objective:To explore the feelings and experiences of female patients with stress urinary incontinence during preoperative self-readiness.Methods:This is a qualitative research. From September to December 2021, a total of 12 female patients with stress urinary incontinence who underwent elective surgery in the Urology Surgery of the First Affiliated Hospital of Zhengzhou University were selected by objective sampling, and semi-structured interviews were conducted. The interview data were sorted and analyzed through Braun's six-step thematic analysis.Results:A total of four themes were extracted including lack of surgical knowledge, high expectation of surgical outcome, heavy psychological burden of surgery, and high demand for external support. The lack of surgical knowledge included three sub-themes, namely, the lack of understanding of informed consent of surgery, the passive acceptance of informed consent information of surgery, and the bias of surgical information recall. The high expectation of surgical outcome included three sub-themes, namely, complete cure of urinary leakage, unrestricted physical and social activities, and getting rid of the negative emotion of "social cancer". The heavy psychological burden of surgery included two sub-themes, namely, concerns about the effects and complications of surgery and the tension of public health events. The high demand for external support included three sub-themes, namely, the need for emotional communication with medical and nursing staff, the need for experience sharing with patients, and the need for emotional support from family members and friends.Conclusions:Medical and nursing staff should pay attention to improving the surgical knowledge of female patients with stress urinary incontinence, respect their autonomy of informed consent, correctly guide patients to establish surgical expectations, and provide them with multi-dimensional emotional support, so as to promote the improvement of patients' preoperative readiness.

Objective:To investigate the status quo of self-care ability of patients after sacral neuromodulation in the first stage, and analyze its influencing factors.Methods:The convenient sampling method was used to select 106 patients after sacral neuromodulation in the first stage who were admitted to Department of Urology in the First Affiliated Hospital of Zhengzhou University from July 2019 to July 2021. Questionnaires were carried out using general information questionnaire, Exercise of Self-Care Agency Scale (ESCA) and Self-efficacy for Chronic Disease 6-Item Scale (SECD6) . The t test, one-way ANOVA, Pearson correlation analysis and multiple linear regression analysis were used to explore the influencing factors of self-care ability. Results:The self-care ability score of the first stage patients after sacral neuromodulation was (80.07±21.39) , including 9 cases (8.5%) with high level, 81 cases (76.4%) with medium level and 16 cases (15.1%) with low level. There were statistically significant differences in the self-care ability scores of patients with different occupational status, medical insurance type, place of residence, residence status, educational level and per capita monthly income of the family ( P<0.05) . The total score and each dimension score of patients' self-care ability were positively correlated with the self-efficacy score ( P<0.05) . The results of multiple linear regression analysis showed that occupational status, educational level, per capita monthly family income and self-efficacy were the main influencing factors of self-care ability of patients after sacral neuromodulation in the first stage ( P<0.05) . Conclusions:The self-care ability of the first stage patients after sacral neuromodulation is generally at a moderate level and the self-care ability of patients needs to be further improved, especially the self-care skills need to be strengthened. Nursing staff should give patients individualized health guidance according to different influencing factors to help them improve their self-care ability.

Acute-on-chronic liver failure (ACLF) is a syndrome characterized by multiple organ failure and high short-term mortality rate, and it has always been a research hotspot in the field of severe liver diseases. Therefore, early and accurate risk stratification and timely intervention are of great significance to improve prognosis. This article summarizes the serum biomarkers identified in recent years for evaluating the prognosis of patients with ACLF, and it is pointed out that new serum biomarkers have an important guiding significance in the prognostic evaluation of ACLF patients.

By reviewing the concept, content, evaluation tools and significance to clinical practice of preoperative patients' preparedness for selective surgery, this paper aims to provide some reference for enriching the content of preoperative patients' preparedness and improving the surgical satisfaction and quality of life for selective surgery.

Objective To study the risk factors of severe complications possibly associated with exchange transfusion (ET) for patients with extreme hyperbilirubinemia.Method From May 2001 to May 2018,neonates with severe complications of ET were assigned into adverse event group.The propensity score principle was used to match adverse event group/the control group with the ratio of 1 ∶ 3.The neonates in the control group received ET without any severe complications.The demographic characteristics of patients,and other clinical data were reviewed.The Logistic regression analysis was used to determine the risk factors of severe complications of ET.Result Among the 1 535 neonates who received ET during the past 17 years,71 neonates (4.6％) were identified with severe adverse events,including apnea (30.6％),necrotizing enterocolitis (18.8％),heart failure (14.1％),respiratory failure (12.9％),and shock (8.2％).The Logistic regression analysis showed that acute bilirubin encephalopathy (ABE) score ≥ 3 (OR=6.383,95％CI 2.550～15.979),ETs ≥ 2 times (OR=11.825,95％CI 2.464～56.755),cardiac murmur ≥ grade Ⅲ (OR=20.417,95％CI 4.705～40.590),and dramatic blood pressure fluctuation during ET ≥ 30 mmHg (OR=13.612,95％CI 1.795～43.342) were risk factors of ET related severe complications (all P＜0.05).Conclusion The indications should be carefully assessed before ET.The patients with ABE score ≥ 3,ETs ≥ 2 times,cardiac murmur ≥ grade Ⅲ,or dramatic blood pressure fluctuation during ET ≥ 30 mmHg should be monitored carefully and ET should be stopped in time if necessary.

Objective To construct a self management program for patients with ureteral stoma, then validate the proposal. Methods The expert consensus reached an agreement after 2 rounds of Delphi expert consultation of "self management programme", according with the contents of the manual requirements,the intervention patients(41 cases)were given these self management interventions:regular one-on-one ostomy skills training, mental support and motivational interview, recording stoma diary, the control patients were given routine nursing interventions,after 1,3 and 6 months of implementation,their results of self management was evaluated. Results The total score of self-management ability was 99.32±10.41,105.34±13.75,109.18±10.91 in the control group after the intervention of 1,3 and 6 months respectively,and 106.73 ± 13.82,117.65 ± 15.47,129.43 ± 12.37 in the test group,the differences were statistically significant (t=7.847, 9.318, 11.416, P<0.01). The total score of quality of life was 167.18 ± 24.62, 173.36 ± 25.33, 179.48 ± 22.17 in the control group after the intervention of 1, 3 and 6 months respectively,and 178.66 ± 23.47,185.41 ± 22.67,213.17 ± 26.75 in the test group,the differences were statistically significant (t=6.839, 10.241, 13.926, P<0.01). Conclusions The construction of the ureterostomy self-management program, enhance patients'self-management consciousness, and improve the ability of self-management.

Objective To discuss the influence of temperature on the effects of cleaning rigid cystoscope. Methods Totally 225 sets of rigid cystoscope which were used for cystoscopy in the Urologic Laparoscopy Center of the First Affiliated Hospital of Zhengzhou University from January 2017 to June 2017 were selected, each of them included four detection points: endoscope, scope lemma, obturator and cross-structure. They were randomly divided into three groups, each group including 75 sets of cystoscope and 300 detection points. The Twist'n Fill'TM cleaner distributor developed by 3M Company was used to control the temperature at 21-30 ℃ for Group A, 31-40 ℃ for Group B and 41-50 ℃ for Group C. The cleaning results were tested through the Clean-Trace ATP Surface Test and ATP Water Test developed by 3M Company. Results The cleaning yields of Group A, B and C were 65.67%, 75.67% and 96.00% respectively, the difference was statistically significant (χ2=86.748; P<0.05). Conclusions Multi-enzymatic detergent shows the best effect at the temperature of 41-50 ℃, and it is advised that this temperature be used in cleaning rigid cystoscope.