Based on the principle of selective photothermodynamics, specific laser can be selectively absorbed by melanin particles in the hair follicles, resulting in a photothermal effect, through which hairball and hair follicle stem cells are completely destroyed, rendering permanent hair removal. Laser and light technology have the characteristics of convenience, efficiency, safety and permanent effect. Since its application in the reconstruction of the outer ear, it has helped solve the problem of residual hair and improve the shape of the auricle and the satisfaction of patients. The authors reviewed the laser types, treatment parameters, timing and interval, as well as related complications and corresponding treatment measures.

Cartilage tissue engineering offers a promising therapeutic approach for auricular, tracheal, and joint reconstruction. However, implanted engineered cartilage currently faces challenges such as host immune rejection and inflammatory reactions, which can lead to deformities and fibrosis, compromising long-term maintenance of shape and function. Effectively modulating immune and inflammatory responses is crucial for advancing the clinical application of engineered cartilage. This article reviewed relevant literature to analyze the mechanisms of inflammatory responses occurring after the implantation of tissue-engineered cartilage, and summarized the types and surface characteristics of biomaterials, providing a reference for the design of biomaterials in cartilage tissue engineering.

The chest deformity result ing from ear reconstruction using autologous costal cartilage significantly impacts patients’ physical development and overall quality of life. In recent years, numerous scholars have conducted extensive research on the diagnostic and therapeutic processes of chest deformities following costal cartilage surgery. The objective of this article is to comprehensively review and summarize the measurement indicators, mechanism, influencing factors, prevention strategies, and research advancements pertaining to chest deformity following costal cartilage surgery. This will serve as a valuable reference for enhancing clinical practices.

Objective:To construct adjustable stiffness hydrogels based on gelatin methacryloyl (GelMA), in order to better simulate the growth environment under physiological conditions.Methods:SYLGARD 184 silicone rubber was used to prepare adjustable stiffness hydrogels. The stiffness values of the adjustable stiffness hydrogels prepared with GelMA of different grafting rates (30%, 60%, 90%) at various concentrations (5%, 10%, 15%) were quantified using Young’s modulus. Based on the result, the optimal grafting rate GelMA was selected as the material for constructing the adjustable stiffness hydrogels. The relationship between the concentration (2%, 3%, 5%, 6%, 8%, 10%, 15%) of 90% grafting rate (GM90) and the stiffness after photopolymerization was determined by measuring the Young’s modulus of GelMA at different concentrations and fitting the data. Three groups of suitable concentrations were chosen to construct three different stiffness hydrogels. After soaking in phosphate-buffered saline for 48 hours, various samples underwent biomechanical measurements, internal pore structure observation, atomic force microscopy to measure parameters such as Young’s modulus, pore size and surface roughness. Rheological tests were carried out, curves were drawn according to the energy storage modulus and loss modulus, and shear frequencies of the intersection points of the two curves were observed at each concentration to evaluate the ability of hydrogel to maintain its morphology. Data analysis was performed using GraphPad Prism 8.0.2 software. Quantitative data were expressed as Mean±SD, and one-way ANOVA was used for comparison among multiple groups, and LSD method was used for pairwise comparison. Statistical significance was set at P<0.05. Results:Increasing the grafting rate and concentration of GelMA hydrogels effectively enhanced their stiffness. GelMA with a GM90 provided a broader range of stiffness, making it suitable for constructing the adjustable stiffness hydrogels. Fitting curves of GM90 concentrations and stiffness after photopolymerization showed that at concentrations of 2%, 6%, and 15%, GelMA could mimic the stiffness of various tissues of human. Therefore, these three concentrations were selected for subsequent experiments. The Young’s modulus of GM90 at concentrations of 15%, 6% and 2% were (98.43±7.71), (14.57±1.62), and (2.11±0.32) kPa, respectively, with statistically significant differences between each pair of groups ( P<0.01). The pore sizes were (41.32±3.51), (3.26±0.33), and (1.21±0.11) μm, respectively, showing significant differences between each pair of groups ( P<0.01). Surface roughness values were (48.15±2.65), (22.65±1.78), and (24.12±1.43) nm, respectively, with 15% concentration showing significantly higher roughness than the 2% and 6% concentrations ( P<0.01), while there was no significant difference between the 2% and 6% concentrations ( P>0.05). Rheological testing indicated that as the concentration decreased, the shear frequency at the crossover point of the storage modulus and loss modulus of GM90 hydrogels decreased, >100, 30 and 18 Hz, respectively. The result showed that the solid form of GM90 hydrogel with 15% concentration was more stable, while the hydrogel with 2% concentration had better fluidity. Conclusion:Adjusting the grafting rate and concentration can regulate the stiffness of GelMA hydrogels. GelMA hydrogels with a 90% grafting rate provide a wide range of stiffness, making them ideal materials for constructing hydrogels with adjustable stiffness. At concentrations of 2%, 6%, and 15%, GM90 exhibited different mechanical properties and internal structures.

Based on the principle of selective photothermodynamics, specific laser can be selectively absorbed by melanin particles in the hair follicles, resulting in a photothermal effect, through which hairball and hair follicle stem cells are completely destroyed, rendering permanent hair removal. Laser and light technology have the characteristics of convenience, efficiency, safety and permanent effect. Since its application in the reconstruction of the outer ear, it has helped solve the problem of residual hair and improve the shape of the auricle and the satisfaction of patients. The authors reviewed the laser types, treatment parameters, timing and interval, as well as related complications and corresponding treatment measures.

Cartilage tissue engineering offers a promising therapeutic approach for auricular, tracheal, and joint reconstruction. However, implanted engineered cartilage currently faces challenges such as host immune rejection and inflammatory reactions, which can lead to deformities and fibrosis, compromising long-term maintenance of shape and function. Effectively modulating immune and inflammatory responses is crucial for advancing the clinical application of engineered cartilage. This article reviewed relevant literature to analyze the mechanisms of inflammatory responses occurring after the implantation of tissue-engineered cartilage, and summarized the types and surface characteristics of biomaterials, providing a reference for the design of biomaterials in cartilage tissue engineering.

The chest deformity result ing from ear reconstruction using autologous costal cartilage significantly impacts patients’ physical development and overall quality of life. In recent years, numerous scholars have conducted extensive research on the diagnostic and therapeutic processes of chest deformities following costal cartilage surgery. The objective of this article is to comprehensively review and summarize the measurement indicators, mechanism, influencing factors, prevention strategies, and research advancements pertaining to chest deformity following costal cartilage surgery. This will serve as a valuable reference for enhancing clinical practices.

Objective:To construct adjustable stiffness hydrogels based on gelatin methacryloyl (GelMA), in order to better simulate the growth environment under physiological conditions.Methods:SYLGARD 184 silicone rubber was used to prepare adjustable stiffness hydrogels. The stiffness values of the adjustable stiffness hydrogels prepared with GelMA of different grafting rates (30%, 60%, 90%) at various concentrations (5%, 10%, 15%) were quantified using Young’s modulus. Based on the result, the optimal grafting rate GelMA was selected as the material for constructing the adjustable stiffness hydrogels. The relationship between the concentration (2%, 3%, 5%, 6%, 8%, 10%, 15%) of 90% grafting rate (GM90) and the stiffness after photopolymerization was determined by measuring the Young’s modulus of GelMA at different concentrations and fitting the data. Three groups of suitable concentrations were chosen to construct three different stiffness hydrogels. After soaking in phosphate-buffered saline for 48 hours, various samples underwent biomechanical measurements, internal pore structure observation, atomic force microscopy to measure parameters such as Young’s modulus, pore size and surface roughness. Rheological tests were carried out, curves were drawn according to the energy storage modulus and loss modulus, and shear frequencies of the intersection points of the two curves were observed at each concentration to evaluate the ability of hydrogel to maintain its morphology. Data analysis was performed using GraphPad Prism 8.0.2 software. Quantitative data were expressed as Mean±SD, and one-way ANOVA was used for comparison among multiple groups, and LSD method was used for pairwise comparison. Statistical significance was set at P<0.05. Results:Increasing the grafting rate and concentration of GelMA hydrogels effectively enhanced their stiffness. GelMA with a GM90 provided a broader range of stiffness, making it suitable for constructing the adjustable stiffness hydrogels. Fitting curves of GM90 concentrations and stiffness after photopolymerization showed that at concentrations of 2%, 6%, and 15%, GelMA could mimic the stiffness of various tissues of human. Therefore, these three concentrations were selected for subsequent experiments. The Young’s modulus of GM90 at concentrations of 15%, 6% and 2% were (98.43±7.71), (14.57±1.62), and (2.11±0.32) kPa, respectively, with statistically significant differences between each pair of groups ( P<0.01). The pore sizes were (41.32±3.51), (3.26±0.33), and (1.21±0.11) μm, respectively, showing significant differences between each pair of groups ( P<0.01). Surface roughness values were (48.15±2.65), (22.65±1.78), and (24.12±1.43) nm, respectively, with 15% concentration showing significantly higher roughness than the 2% and 6% concentrations ( P<0.01), while there was no significant difference between the 2% and 6% concentrations ( P>0.05). Rheological testing indicated that as the concentration decreased, the shear frequency at the crossover point of the storage modulus and loss modulus of GM90 hydrogels decreased, >100, 30 and 18 Hz, respectively. The result showed that the solid form of GM90 hydrogel with 15% concentration was more stable, while the hydrogel with 2% concentration had better fluidity. Conclusion:Adjusting the grafting rate and concentration can regulate the stiffness of GelMA hydrogels. GelMA hydrogels with a 90% grafting rate provide a wide range of stiffness, making them ideal materials for constructing hydrogels with adjustable stiffness. At concentrations of 2%, 6%, and 15%, GM90 exhibited different mechanical properties and internal structures.

OBJECTIVE To investigated the anti-de-pressant effects of the fruit Areca catechu L.(ACL)and elucidated its potential underlying mechanism using a rat model of chronic unpredictable mild stress(CUMS).METHODS CUMS was induced in rats to establish a depression animal model for 28 d.According to the baseline sucrose preference,the male rats were divided into six different groups.They were treated with parox-etine hydrochloride,ACL,and water once a day until the behavioral tests were performed.The levels of corticoste-rone(CORT),malondialdehyde(MDA),catalase(CAT),and total superoxide dismutase(T-SOD)in serum were de-tected using a commercial kit,and the concentrations of 5-hydroxytryptamine(5-HT)and dopamine(DA)mono-amine neurotransmitters in the brain tissues were detect-ed by liquid chromatography-tandem mass spectrometry.Doublecortin(DCX)expression in the hippocampal den-tate gyrus(DG)was determined by immunofluorescence,and the relative abundance of brain-derived neurotrophic factor(BDNF),TrkB,PI3K,p-Akt/Akt,PSD-95,and p-GSK-3β/GSK-3β of brain tissues were assayed by West-ern blotting.RESULTS ACL markedly increased sucrose preference,decreased the immobility time,and short-ened the feeding latency of CUMS-induced rats.CUMS induction resulted in marked changes in the contents of the monoamine neurotransmitters(5-HT and DA)in the hippocampus and cortex of brain tissues and the levels of CORT,MDA,CAT,and T-SOD in serum,whereas ACL administration alleviated these considerable changes.ACL promoted DCX expression in DG and increased the protein levels of BDNF,TrkB,PI3K,p-Akt/Akt,PSD-95,and p-GSK-3β/GSK-3β in the brains of CUMS-induced rats.CONCLUSION Our results indicated that ACL may improve depression-like behaviors in CUMS-induced rats by decreasing the hyperfunction and oxidative stress of the hypothalamic-pituitary-adrenal axis,stimulating hippo-campal neurogenesis,and activating the BDNF signaling pathway.

The aesthetic effect of ear reconstruction depends on a fine reconstruction of the auricle and a good projection of the structure. Constructing a stable auriculo-cephalic angle is one of the primary points to obtain a symmetrical and three-dimensional auricle, making reconstructing a more precise auriculo-cephalic angle a hotspot in this field and has made further progress in recent years. The selection of the supporting framework and the covering tissue, the application of digital technology and tissue engineering technology in the modified ear elevation are reviewed in this article.