1.Community-driven evaluation of E. coli levels in domestic-use underground water sources in Angono, Philippines.
Hisaaki KATO ; Seiji MARUYAMA ; Gilbert J. MERINO ; Noel R. JUBAN
Acta Medica Philippina 2022;56(3):6-17
Objectives: The authors considered the community-based on-site water sampling-surveys case-study in Angono, Rizal, in the Republic of the Philippines. The study was designed to determine whether individual citizens within the community could successfully collaborate in order to develop a method for quantification of E. coli levels in water (especially underground water) for domestic use.
Methods: We used techniques that had been validated in Japan for on-site evaluation of E. coli. These simple techniques enabled the on-site quantification of E. coli levels in multiple water sources, including underground water.
Results: The techniques used in Japan were successfully adopted in the Philippines, and we cooperated with regional medical technicians, scientists, and citizens, in order to develop a system where ultimately, the people in Angono can perform all the tasks related to on-site water quality evaluation. From societal and economic perspectives, we were able to reduce the traditionally high laboratory cost (including testing cost and samples transit cost) of coliform bacteria and E. coli evaluation to a lower, fixed cost level that is affordable for regional health centers and other sites.
Conclusion: The transfer of a simple inspection method technology, under the supervision of an experienced Japanese research team, allowed us to validate the E. coli on-site quantification strategy at a regional level as "co-creation of science and society". In addition, beyond technology transfer, our collaborative efforts with scientists, regional medical engineers, and citizens, led to the creation of an NGO (non-governmental organization) that serves citizens in the town of Angono with regard to water quality assessment and certification activities.
Key Words: water quality, water management, community, local people, participatory approach, underground water, Escherichia coli, coliform bacteria, Philippines
Water Quality ; Residence Characteristics ; Groundwater
2.Bibliometric analysis on bioremediation of organic contaminated soil and groundwater based on Web of Science database.
Qing WANG ; Zongshuai YANG ; Lipu YIN ; Xin SONG ; Changlong WEI ; Yanli LI ; Wei ZHAI
Chinese Journal of Biotechnology 2021;37(10):3549-3564
		                        		
		                        			
		                        			Bioremediation is regarded as one of the most promising methods for the remediation of the soil and groundwater contaminated with organic compounds, mainly due to its cost-effectiveness and environmental friendliness. Using VOSviewer and CiteSpace, we analyzed all publications in this area in core database of Web of Science from 1990 to 2020. The number of publications in this area showed an increasing trend worldwide. The country with the largest total number of publications was USA, followed by China and India. Since 2012, the number of annual publications of China exceeded USA and ranked the first. The top three journals which published papers in this area were Chemosphere, Environmental Science & Technology, and Science of the Total Environment. The Chinese Academy of Sciences published the largest number of papers among the research institutions globally, but the University of California in USA had the highest total citations and h-index. Bibliometric analysis indicated that it is important to develop biotechnologies of bioremediation coupled with chemical/physical remediation to overcome the challenge of low efficiency and long remediation duration associated with bioremediation. In addition, the bioremediation on the mixed contaminants, such as organic contaminants and heavy metals, or mixtures of different organic contaminants, is an important direction. Finally, the rapid development of microbiome technologies will further promote the exploration of mechanisms involved in bioremediation on the genetic and molecular level.
		                        		
		                        		
		                        		
		                        			Bibliometrics
		                        			;
		                        		
		                        			Biodegradation, Environmental
		                        			;
		                        		
		                        			Groundwater
		                        			;
		                        		
		                        			Publications
		                        			;
		                        		
		                        			Soil
		                        			
		                        		
		                        	
4.Peripheral neuropathy induced by drinking water contaminated with low-dose arsenic in Myanmar.
Hitoshi MOCHIZUKI ; Khin Phyu PHYU ; Myo Nanda AUNG ; Phyo Wai ZIN ; Yasunori YANO ; Moe Zaw MYINT ; Win Min THIT ; Yuka YAMAMOTO ; Yoshitaka HISHIKAWA ; Kyaw Zin THANT ; Masugi MARUYAMA ; Yoshiki KURODA
Environmental Health and Preventive Medicine 2019;24(1):23-23
		                        		
		                        			BACKGROUND:
		                        			More than 140 million people drink arsenic-contaminated groundwater. It is unknown how much arsenic exposure is necessary to cause neurological impairment. Here, we evaluate the relationship between neurological impairments and the arsenic concentration in drinking water (ACDW).
		                        		
		                        			PARTICIPANTS AND METHODS:
		                        			A cross-sectional study design was employed. We performed medical examinations of 1867 residents in seven villages in the Thabaung township in Myanmar. Medical examinations consisted of interviews regarding subjective neurological symptoms and objective neurological examinations of sensory disturbances. For subjective neurological symptoms, we ascertained the presence or absence of defects in smell, vision, taste, and hearing; the feeling of weakness; and chronic numbness or pain. For objective sensory disturbances, we examined defects in pain sensation, vibration sensation, and two-point discrimination. We analyzed the relationship between the subjective symptoms, objective sensory disturbances, and ACDW.
		                        		
		                        			RESULTS:
		                        			Residents with ACDW ≥ 10 parts per billion (ppb) had experienced a "feeling of weakness" and "chronic numbness or pain" significantly more often than those with ACDW < 10 ppb. Residents with ACDW ≥ 50 ppb had three types of sensory disturbances significantly more often than those with ACDW < 50 ppb. In children, there was no significant association between symptoms or signs and ACDW.
		                        		
		                        			CONCLUSION
		                        			Subjective symptoms, probably due to peripheral neuropathy, occurred at very low ACDW (around 10 ppb). Objective peripheral nerve disturbances of both small and large fibers occurred at low ACDW (> 50 ppb). These data suggest a threshold for the occurrence of peripheral neuropathy due to arsenic exposure, and indicate that the arsenic concentration in drinking water should be less than 10 ppb to ensure human health.
		                        		
		                        		
		                        		
		                        			Adolescent
		                        			;
		                        		
		                        			Adult
		                        			;
		                        		
		                        			Arsenic
		                        			;
		                        		
		                        			analysis
		                        			;
		                        		
		                        			toxicity
		                        			;
		                        		
		                        			Cross-Sectional Studies
		                        			;
		                        		
		                        			Dietary Exposure
		                        			;
		                        		
		                        			adverse effects
		                        			;
		                        		
		                        			Dose-Response Relationship, Drug
		                        			;
		                        		
		                        			Drinking Water
		                        			;
		                        		
		                        			adverse effects
		                        			;
		                        		
		                        			chemistry
		                        			;
		                        		
		                        			Female
		                        			;
		                        		
		                        			Groundwater
		                        			;
		                        		
		                        			chemistry
		                        			;
		                        		
		                        			Humans
		                        			;
		                        		
		                        			Male
		                        			;
		                        		
		                        			Middle Aged
		                        			;
		                        		
		                        			Myanmar
		                        			;
		                        		
		                        			epidemiology
		                        			;
		                        		
		                        			Peripheral Nervous System Diseases
		                        			;
		                        		
		                        			chemically induced
		                        			;
		                        		
		                        			epidemiology
		                        			;
		                        		
		                        			physiopathology
		                        			;
		                        		
		                        			Sensation Disorders
		                        			;
		                        		
		                        			chemically induced
		                        			;
		                        		
		                        			epidemiology
		                        			;
		                        		
		                        			physiopathology
		                        			;
		                        		
		                        			Water Pollutants, Chemical
		                        			;
		                        		
		                        			analysis
		                        			;
		                        		
		                        			toxicity
		                        			;
		                        		
		                        			Young Adult
		                        			
		                        		
		                        	
5.An Norovirus Outbreak at a Local Festival in Chungnam Korea
Hyunah LEE ; Donguk KIM ; Seongmin PARK ; Jongjin PARK ; Hae Sung NAM ; Jinha CHOI ; Junhyuk PARK
Journal of Bacteriology and Virology 2019;49(2):81-88
		                        		
		                        			
		                        			Noroviruses (NoV) are the major viral pathogen causing epidemic acute gastroenteritis and outbreaks of foodborne and waterborne illness. During the local festival in Chungnam province, group food poisoning occurred outbreak by NoV infections in Jan 2019. In this study, epidemiological analysis and molecular characterization were conducted such as genotyping, phylogeny. The prevalent genotypes of food poisoning events were NoV GII.3 and GII.17, and NoV GII.3 and GII.17 isolates of this study were completely matched in nucleotide sequence comparison of capsid gene region, respectively. In underground water and stream water, various multiple genotypes of noroviruses were detected including NoV GII.3, GII.8 and GI.4 in aquatic environment of the local festival site. Among 32 worker samples, various NoVs of five genotypes (GI.7, GI.8, GII.3, GII.8, GII.17) were detected in 12 samples and expected to causing NoV contaminated by exposure to groundwater. NoV genotype GII.3, which was detected from groundwater 2, was completely consistent with that of patients and workers. Therefore, groundwater within the local festival site could be main cause of food poisoning event. Because NoV outbreaks are caused by fecal to oral transmission, proper management of sewage purification facilities, groundwater and sanitary toilets is required for many visitors, and efforts are needed to maintain clean environment.
		                        		
		                        		
		                        		
		                        			Base Sequence
		                        			;
		                        		
		                        			Capsid
		                        			;
		                        		
		                        			Chungcheongnam-do
		                        			;
		                        		
		                        			Disease Outbreaks
		                        			;
		                        		
		                        			Epidemiologic Studies
		                        			;
		                        		
		                        			Foodborne Diseases
		                        			;
		                        		
		                        			Gastroenteritis
		                        			;
		                        		
		                        			Genotype
		                        			;
		                        		
		                        			Groundwater
		                        			;
		                        		
		                        			Holidays
		                        			;
		                        		
		                        			Humans
		                        			;
		                        		
		                        			Korea
		                        			;
		                        		
		                        			Norovirus
		                        			;
		                        		
		                        			Phylogeny
		                        			;
		                        		
		                        			Rivers
		                        			;
		                        		
		                        			Sewage
		                        			;
		                        		
		                        			Water
		                        			
		                        		
		                        	
6.Fate and toxicity of spilled chemicals in groundwater and soil environment I: strong acids
Doyun SHIN ; Youngyeon KIM ; Hee Sun MOON
Environmental Health and Toxicology 2018;33(4):2018019-
		                        		
		                        			
		                        			We reviewed the chemical/physical properties, toxicity, environmental fate, and ecotoxicity of strong acids in soil and groundwater environments. We recommend that sulfuric acid and hydrofluoric acid be classified as chemicals of priority control based on volumes used, toxicity, carcinogenicity, and past significant spill events. Understanding the behavior and transport of spilled strong acids in soil and groundwater environments requires a multi-disciplinary approach, as they can undergo a variety of geochemical and biochemical reactions with complex geomedia. The toxicity of spilled acid is dependent on the characteristics of the geomedia exposed to the acid and the amount of residual protons following acid–substrate interaction. Soil texture, cation exchange capacity, mineral composition, bedrock type, and aluminum content may be important factors affecting the toxicity of spilled acid in soil-groundwater environments. We expect that the results of this study will contribute preliminary data for future research on chemical spills.
		                        		
		                        		
		                        		
		                        			Aluminum
		                        			;
		                        		
		                        			Groundwater
		                        			;
		                        		
		                        			Hydrofluoric Acid
		                        			;
		                        		
		                        			Miners
		                        			;
		                        		
		                        			Protons
		                        			;
		                        		
		                        			Soil
		                        			;
		                        		
		                        			Sulfur
		                        			
		                        		
		                        	
7.Fate and toxicity of spilled chemicals in groundwater and soil environment I: strong acids.
Doyun SHIN ; Youngyeon KIM ; Hee Sun MOON
Environmental Health and Toxicology 2018;33(4):e2018019-
		                        		
		                        			
		                        			We reviewed the chemical/physical properties, toxicity, environmental fate, and ecotoxicity of strong acids in soil and groundwater environments. We recommend that sulfuric acid and hydrofluoric acid be classified as chemicals of priority control based on volumes used, toxicity, carcinogenicity, and past significant spill events. Understanding the behavior and transport of spilled strong acids in soil and groundwater environments requires a multi-disciplinary approach, as they can undergo a variety of geochemical and biochemical reactions with complex geomedia. The toxicity of spilled acid is dependent on the characteristics of the geomedia exposed to the acid and the amount of residual protons following acid–substrate interaction. Soil texture, cation exchange capacity, mineral composition, bedrock type, and aluminum content may be important factors affecting the toxicity of spilled acid in soil-groundwater environments. We expect that the results of this study will contribute preliminary data for future research on chemical spills.
		                        		
		                        		
		                        		
		                        			Aluminum
		                        			;
		                        		
		                        			Groundwater*
		                        			;
		                        		
		                        			Hydrofluoric Acid
		                        			;
		                        		
		                        			Miners
		                        			;
		                        		
		                        			Protons
		                        			;
		                        		
		                        			Soil*
		                        			;
		                        		
		                        			Sulfur
		                        			
		                        		
		                        	
8.Health Risk Associated With Aluminium Exposure In Groundwater: A Cross-Sectional Study In An Orang Asli Village In Jenderam Hilir, Selangor, Malaysia
Malaysian Journal of Public Health Medicine 2017;2017(Special Volume (1)):58-62
		                        		
		                        			
		                        			The purpose of this study was to determine aluminium (Al) concentrations in groundwater used for drinking and cooking and its related health risk among population of 28th Mile Orang Asli village in Jenderam Hilir, Selangor, Malaysia. A total of 100 respondents were recruited, comprising 51 (51.0 %) male and 49 (49.0 %) female residents. Inductively coupled plasma mass spectrometry (ICP-MS) was used to determine Al concentration, while the LAMOTTE TRACER ORP PockeTester was used to measure pH levels. Statistical Package for Social Science (SPSS) was used to analyze the data. Results showed that Al concentration ranged from 0.041 - 0.136 mg/L with a mean of 0.136 + SD 0.041 mg/L, hence the values obtained were below the standard value (0.2 mg/L). pH levels ranged from 3.82 to 5.84, with a mean of 4.163 + SD 0.411, which is acidic and below the range permitted by the health authorities. The acidic nature may have an impact on the Al concentration in the water. The Hazard Index (HI) was found to be less than 1, thus there was no health risk of Al exposure in drinking water for the respondents involved. The study area was considered safe from having health risk associated with Al exposure.
		                        		
		                        		
		                        		
		                        			Aluminium
		                        			;
		                        		
		                        			 Ph
		                        			;
		                        		
		                        			 drinking water
		                        			;
		                        		
		                        			 groundwater
		                        			;
		                        		
		                        			 Hazard Index
		                        			
		                        		
		                        	
9.Beneficial effects of natural Jeju groundwaters on lipid metabolism in high-fat diet-induced hyperlipidemic rats.
Yan Chao WANG ; Jin Miao LU ; Hui Zi JIN ; Ai Niu MA ; Jin Yang ZHANG ; Nian GONG ; Qi XIAO ; Bin ZHU ; Ying Fang LV ; Na YU ; Wei Dong ZHANG ; Yong Xiang WANG
Nutrition Research and Practice 2014;8(2):165-171
		                        		
		                        			
		                        			BACKGROUND: Groundwater is believed to possess many beneficial effects due to its natural source of various minerals. In this study, we examined the effects of natural Jeju groundwater S1 (Samdasoo(TM)), S2 and S3 pumped up from different locations of Jeju Island, Korea, along with local tap water, on body weight gain, serum lipids and lipoproteins, and liver histopathology in high-fat diet-induced hyperlipidemic rats. MATERIALS/METHODS: Rats were randomly and equally divided into 6 groups. Different water samples were supplied to the hyperlipidemic rats as their daily drinking water and the widely-used anti-hyperlipidemic drug simvastatin was used as a positive control. Body weight, serum lipids and lipoproteins were measured weekly. Liver weight, liver index and liver histopathology were examined after the execution of the rats. RESULTS: After drinking Jeju groundwaters for two months, S2 but not S3 significantly reduced weight growth and serum triglycerides levels and increased high density lipoprotein-C (HDL-C) without affecting total cholesterol or LDL-C. S1 and particularly S2 significantly reduced the severity of liver hypertrophy and steatosis. All Groundwaters had much higher contents of vanadium (S3>S2>S1>>tap water) whereas S1 and S2 but not S3 markedly blocked autoxidation of ferrous ions. CONCLUSION: Jeju Groundwater S1 and particularly S2 exhibit protective effects against hyperlipidemia and fatty liver and hypothesize that the beneficial effect of Jeju Groundwaters may be contributed from blockade of autoxidation of ferrous ions rather than their high contents of vanadium.
		                        		
		                        		
		                        		
		                        			Animals
		                        			;
		                        		
		                        			Body Weight
		                        			;
		                        		
		                        			Cholesterol
		                        			;
		                        		
		                        			Drinking
		                        			;
		                        		
		                        			Drinking Water
		                        			;
		                        		
		                        			Fatty Liver
		                        			;
		                        		
		                        			Groundwater*
		                        			;
		                        		
		                        			Hyperlipidemias
		                        			;
		                        		
		                        			Hypertrophy
		                        			;
		                        		
		                        			Ions
		                        			;
		                        		
		                        			Korea
		                        			;
		                        		
		                        			Lipid Metabolism*
		                        			;
		                        		
		                        			Lipoproteins
		                        			;
		                        		
		                        			Liver
		                        			;
		                        		
		                        			Minerals
		                        			;
		                        		
		                        			Rats*
		                        			;
		                        		
		                        			Simvastatin
		                        			;
		                        		
		                        			Triglycerides
		                        			;
		                        		
		                        			Vanadium
		                        			;
		                        		
		                        			Water
		                        			
		                        		
		                        	
10.Study of the Detection of Enteric Viruses and Bacteria in Spring-water and Groundwater in Busan ('10~'11).
Seoung Hwa CHOI ; Jae Eun JEONG ; Na Na YUN ; Nam Ho KIM ; Yon Koung PARK ; Eun Young JUNG
Journal of Bacteriology and Virology 2013;43(2):131-139
		                        		
		                        			
		                        			We analyzed the occurrence of enteric viruses and bacteria at 22 places of drinkable groundwater (civil defense emergency water-supply facility), 8 places of the groundwater used for drinking water in group food services, and 10 places of spring-water. When the 40 concentrated samples were analyzed using nested RT-PCR and real-time RT PCR methods, norovirus and other enteric viruses were not detected in all samples tested. The detection percentages for total coliforms, Escherichia coli, Yersinia enterocolitica of fecal indicator were 57.5%, 22.5% and 7.5%, respectively. Colipages were not detected. These results suggest that high levels of fecal indicator bacteria in groundwater and spring-water are not directly related to occurrence of enteric viruses.
		                        		
		                        		
		                        		
		                        			Bacteria
		                        			;
		                        		
		                        			Drinking Water
		                        			;
		                        		
		                        			Emergencies
		                        			;
		                        		
		                        			Escherichia coli
		                        			;
		                        		
		                        			Food Services
		                        			;
		                        		
		                        			Groundwater
		                        			;
		                        		
		                        			Norovirus
		                        			;
		                        		
		                        			Polymerase Chain Reaction
		                        			;
		                        		
		                        			Yersinia enterocolitica
		                        			
		                        		
		                        	
            

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