Objective:To form the expert consensus on nursing care of adults with status epilepticus (SE), promote nurses to provide standardized and safe first aid, monitoring and support to SE patients, and effectively prevent and control complications, with a view to controlling seizures as soon as possible.Methods:The medical and nursing evidence on SE was retrieved, evaluated and summarized, and the retrieval time limit was from the establishment of the database to March 31, 2022. Recommendations and studies were extracted to form a first draft of consensus. A total of 31 experts were selected to conduct two rounds of Delphi expert consultation and expert demonstration meetings to analyze, revise and improve the experts' suggestions and form a consensus final draft.Results:The expert positive coefficient was 100.00% (31/31), the expert judgment coefficient was 0.93, the familiarity degree was 0.87, and the authority coefficient was 0.90. Kendall's harmony coefficient was 0.25 to 0.27 ( P<0.05). The final consensus included adult SE identification, first aid, monitoring and support, complication prevention and control, and effect evaluation of terminating SE. Conclusions:This consensus is practical and provides guidance for clinical nursing practice and quality control of SE patients.

The acupuncture method of Shugan-Tiaoshen is proposed by the famous old Chinese medicine Professor Shan Qiuhua in Shandong Province in combination with Traditional Chinese Medicine theory and clinical experience. Shugan-Tiaoshen acupuncture method is widely used in clinic and has curative effect. It can treat mental diseases, nervous system diseases, digestive system diseases, endocrine system diseases, urinary system diseases, diseases caused by physical and chemical factors and other internal diseases, as well as surgical diseases such as skin trauma, facial symptoms and breast diseases. It can also treat gynecological diseases. It can also improve the brain function of stress disorder. The role of soothing liver and regulating spirit in clinic is never underestimated. In clinical practice, doctors should try their best to combine regulating spirit with treating spirit. Shugan-Tiaoshen acupuncture provides a good idea for clinical treatment, but there are still some problems in the current researches, such as the lack of multi-center, large sample trials, or well-design mechanism researches.

Objective The aim of this study was to determine whether active part of Glycyrrhiza extract (GL)induced apoptosis in Hela cells and its inhibitory mechanism.Methods HeLa cells were treated with 25 g/mL of GL for 24hs.Cell viability and apoptosis in HeLa cells were determined by MTT,AO/EB fluorescent double staining,transmission electron microscope(TEM),and Western blot.Results The MTT results showed that GL significantly inhibited the proliferation of HeLa cells in a dose-response.After treatment for 24 hrs,large number of early apoptotitc cell were observed using AO/EB fluorescent double staining and TEM.The expression of Pro-caspase-9 and Cleaved-caspase-3 protein was higher in GL-treated cells them those of the control cells(P<0.05).Conclusion GL can activate Caspase-3 and Caspase-9 genes to induce apoptosis in HeLa cells.

Objective To analyze the preventive effect of the targeted nursing intervention on lower limb venous thrombosis in patients with hemiplegia after stroke.Methods A total of 120 stroke cases from October 2014 to December 2015 in department of neurology in our hospital were divided into control group and intervention group according to random number table method.The control group was given routine nursing,and the intervention group was given targeted nursing intervention on the basis of the control group.After nursing,the compliance rate of two groups before and after nursing,mastery of disease related knowledge,the incidence of lower limb venous thrombosis and nursing effect were compared.Results There was no significant difference in medication compliance between the two groups before nursing intervention (P ＞ 0.05).In the intervention group,the correct medication,reasonable diet,regular physical examination,smoking cessation,functional exercise,mood control compliance rate were significantly improved,and there was significant difference compared with the control group (P ＜ 0.05).The awareness rate of the intervention group was significantly higher than that of the control group (P ＜ 0.05).The nursing effective rate was significantly higher in the intervention group than that of the control group,(43.10％ vs.85.94％,P ＜ 0.05).There were 3 cases(13.79％) with deep vein thrombosis in the control group,and 1case(1.61％) in the intervention group,there was significant difference in deep vein thrombosis (P ＜ 0.05).Conclusion The nursing intervention of department of neurology for stroke patients can significantly improve the compliance rate and disease related knowledge,reduce the incidence of venous thrombosis,improve the nursing effect,so it is worthy of promotion in clinical nursing work.

Objective To analyze the preventive effect of the targeted nursing intervention on lower limb venous thrombosis in patients with hemiplegia after stroke.Methods A total of 120 stroke cases from October 2014 to December 2015 in department of neurology in our hospital were divided into control group and intervention group according to random number table method.The control group was given routine nursing,and the intervention group was given targeted nursing intervention on the basis of the control group.After nursing,the compliance rate of two groups before and after nursing,mastery of disease related knowledge,the incidence of lower limb venous thrombosis and nursing effect were compared.Results There was no significant difference in medication compliance between the two groups before nursing intervention (P ＞ 0.05).In the intervention group,the correct medication,reasonable diet,regular physical examination,smoking cessation,functional exercise,mood control compliance rate were significantly improved,and there was significant difference compared with the control group (P ＜ 0.05).The awareness rate of the intervention group was significantly higher than that of the control group (P ＜ 0.05).The nursing effective rate was significantly higher in the intervention group than that of the control group,(43.10％ vs.85.94％,P ＜ 0.05).There were 3 cases(13.79％) with deep vein thrombosis in the control group,and 1case(1.61％) in the intervention group,there was significant difference in deep vein thrombosis (P ＜ 0.05).Conclusion The nursing intervention of department of neurology for stroke patients can significantly improve the compliance rate and disease related knowledge,reduce the incidence of venous thrombosis,improve the nursing effect,so it is worthy of promotion in clinical nursing work.

The myelodysplastic syndrome (MDS) is a clonal disorder characterized by inefficient haematopoiesis,dysplasia of hematopoietic cells in bone marrow and unable production of mature cells with normal differentiation resulting in peripheral cytopenias.The incidence of MDS is increased with the increasing age,suggesting that the accumulation of genetic or epigenetic changes lead to DNA mutations in hematopoietic stem cell,activation of oncogenes and inactivation of tumor suppressor genes,increasing limitless self-proliferation,and eventually resulting in aberrant clonal hematopoiesis and the occurrence of MDS.About thirty percentage of patients with MDS will transform into acute myeloid leukemia (AML) at last.MDS is always not sensitive to cytotoxic drugs,but targeted drugs maybe help to improve the prognosis.

JAK2 V617F gene mutation positive myeloproliferative neoplasms (MPN) consists of polycythemia vera (PV),essential thrombocythemia (ET) and primary myelofibrosis (PMF).This article focuses on their risk scoring systems and treatment including first-and second-line therapies,JAK2 inhibitors,cytoreduction,antifibrosis and other single-agent or combination therapy.

Outpatient service constitutes a key part of hospital services.In an effort to optimize services plagued by the too long waiting time in registration,payment and drug dispensary,and the too short time in seeing doctors,Beijing Friendship Hospital introduced mobile Intemet technology into hospital outpatient services.Such measures as mobile payment,and realtime push of the information of patients waiting were adopted to help with outpatients waiting at home,minimize outpatients' movement,queuing and waiting.Simplified service process and higher service efficiency proved effective in winning higher outpatient satisfaction as a result.

The paper introduced the attempts made by the hospital in the ongoing health reform.The hospital encouraged incentives of the medical workers to take part in the reform,as they upgrade their work in high quality nursing care,outpatient appointment registration,better specialist outpatient clinic service,and tiered medical services.These efforts help innovate medical services for fulfillment of goals of the health reform.

Objective To investigate the expression and significance of breast cancer suscepterbility gene 1 (BRCA1) in leukemia.Methods Fluorescent quantitative reverse transcription-polymerase PCR was used to investigate the expression of BRCA1 in 18 patients with ALL-L2 (13 denovo ALL patients,5 relapsed ALL patients),20 patients with CML-CP and 15 normal controls.Results The mRNA expression of BRCA1 in denovo patients with ALL was lower than that in normal control,with statistical significance (P ＜ 0.05).The mRNA level of BRCA1 in ALL patients in CR was higher compared with before to cure, with statistical significance (P ＜ 0.05),but lower than that in normal control,without statistical significance(P ＞ 0.05).The mRNA expression of BRCA1 in patients with relapsed ALL was lower than that in normal control with statistical significance (P ＜ 0.05), and lower than that in denovo patients with ALL, without statistical significance (P ＞ 0.05).The mRNA level of BRCA1 showed no difference in CML-CP patients compared with normal control (P ＞ 0.05). Conclusion The different expression of BRCA1 in leukemia indicates that he has closely relationship with the prognosis of leukemia and guides the clinical diagnosis and treatment.