Orthodontic moving teeth are closely related to the health of periodontal support tissues, and the combined periodontal orthodontic treatment is becoming more and more normalized. Inflammation control is the prerequisite for orthodontic treatment in patients with periodontitis. For many adult patients, the long course of treatment and the risk of periodontal tissue damage during orthodontic treatment have gradually attracted the clinicians′ attention. Periodontally accelerated osteogenic orthodontics (PAOO) is one of the surgical strategies by combining selective decortication with alveolar augmentation to promote orthodontic treatment. It is aiming to accelerate the movement of teeth for shortening the treatment course, to increase the bone volume for reshaping the alveolar bone and expanding the range of tooth movement and to reduce the risk of tooth movement beyond the "alveolar bone boundary" during orthodontic treatment. Based on the retrieve and review of current literature, this article will focus on how to design and implement PAOO surgery so as to maximize the effectiveness in clinical practice.

Objective? To compare the difference in psychological resilience and professional identity of nursing students with different temperaments and to provide a reference for providing personalized psychological and vocational counseling. Methods? Nursing students who practiced in Zhengzhou University Affiliated Tumor Hospital between May 2015 and March 2017 were selected using convenient sampling and investigated with the general information questionnaire, temperament scale, psychological resilience scale and nurses' professional identity scale. Totally 380 questionnaires were distributed, and 368 valid questionnaires were collected. Results? The temperaments of the 368 nursing students were choleric (63), sanguineous (109), phlegmatic (77), melancholic (36) and mixed (83). Their professional identity and physical resilience scores totaled (56.0±12.0) and (61.7±10.3), respectively. There were statistical differences in the total score and in difference dimensions of psychological resilience of different temperament types (P＜ 0.05). There were statistical differences in the total score of professional identity and in the dimensions of professional self-concept, social comparison and self-reflection and social persuasion of different temperament types (P＜0.05). Conclusions? Nursing students' psychological resilience and professional identity stands at a medium and low level, and there is difference in psychological resilience and professional identity in nursing students of different temperaments. Nursing educators shall provide targeted psychological and vocational counseling based on nursing students' temperaments.

Objective To explore the experience of nurse-physician collaboration construction and its clinical practice effects. Methods From June 2013 to December 2015, based on the SBAR communication model, nurse-physician collaboration was constructed in oncology and surgery wards of Affiliated Cancer Hospital of Zhengzhou University, He'nan Cancer Hospital. The situation of nurse-physician collaboration, satisfaction of doctors, nurses, patients to nursing work were evaluated using the convenience sampling method and questionnaire survey method, before and after the implementation of nurse-physician collaboration mode. Results After the application, the score of Nurse-Physician Collaboration Scale (NPCS) was (114.89±14.56), which was higher than before (94.37±16.21), and the difference was statistically significant (t=-8.75, P<0.01).The satisfaction degrees about nursing work of physicians, nurses and patients were (93.57±6.43), (95.50±7.89) and (94.80±6.72), which were all higher than before, and the differences were statistically significant (t=-2.07,-5.81,-7.02;P<0.05). Conclusions Based on the SBAR communication mode, the establishment of nurse-physician collaboration mode could effectively promote the degree of cooperation between physicians and nurses, improve the satisfaction of physicians, nurses and patients, and improve the hospital care quality.

Objective:To study the expression of B-1a cells in mice with obesity and periodontal infection.Methods:Mouse models of diet induced obesity combined with experimental periodontitis were established,the expression of CD5 protein,anti-collagenⅠ antibody(anti-Col-Ⅰantibody) and IL-10 protein was examined in mouse jaw bone and spleen by immunohistochemistry and Western blot;The mRNA expression of CD5,anti-Col-Ⅰantibody and IL-10 in mouse jaw bone was detected by real time quantitative PCR.Results:The mRNA and protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in jaw bone in periodontitis group were significantly higher than those in control group(P<0.001).The protein expressions of CD5 and IL-10 and anti-Col-Ⅰantibody in spleen in obesity group were significantly higher than those in standard group(P<0.05).The protein expression of anti-Col-Ⅰantibody in spleen in standard accompanying periodontal ligature group was significantly higher than that in standard without periodontal ligature group(P<0.05).Conclusion:B-1a cells are activated in the early stage of obesity and periodontal inflammation with a certain pathological significance and without interation between the two inflammatory states in the pathological mechanism.

BACKGROUND:Hard palate mucosa serves as a main donor material in periodontal plastic surgery and its thickness is crucial for the surgical outcomes. OBJECTIVE: To analyze the thickness of hard palate mucosa in Han population, and analyze the consistency between cone-beam CT image analysis and trans-gingival probing method. METHODS: A total of 30 Han volunteers (300 teeth) were recruited, and the thickness of hard palate mucosa was measured using cone-beam CT image analysis or trans-gingival probing method, to analyze their consistency. RESULTS AND CONCLUSION: The two methods showed a higher consistency in the thickness of hard palate mucosa at the cuspid, first and second premolars as well as first and second molars. The thickness of the hard palate mucosa related to the distance from the gingival margin and tooth position, the thickness from the canine region to the second premolar region thickening gradually, and became the thickest at the second molar, and the thinnest at the cuspid. This study for the first time analyzed the thickness of hard palate mucosa in Chinese Han population, and confirmed there is a high consistency between cone-beam CT image analysis and trans-gingival probing method.

Objective To investigate the current status of emotional labor of emergency nurses in tertiary general hospitals,and explore the effects of nursing work environment on emotional labor of emergency nurses,so as to provide evidence for emotional management of emergency nurses.Methods Totally 376 emergency nurses in tertiary general hospitals of Zhengzhou were selected by multiple stage sampling method from February 2014 to November 2014,and they were investigated by nurse′s emotional labor scale and nursing work environment scale.Results The total score of nursing work environment of emergency nurses in tertiary general hospitals was(78.82±9.45),and the average score of emotional labor was(53.06±7.10),which was above the middle level. We took the score of emotional labor as dependent variable,the general information of emergency nurses and nursing work environment as the independent variables,then we conducted a regression analysis. The results showed that:education background,frequency of night shift,high quality nursing service and medical cooperation could predict 20.1% of the variance of emotional labor.Conclusions In order to improve nurses′ control ability of emotion,the nursing working conditions of emergency department should be improved,so that the nurses can obtain a good working environment.

OBJECTIVETo explore the role of platelet-activating factor receptor (PAFR) in adhesion and invasion of phospho- rylcholine (PC)-positive Aggregatibacter actinomycetemcomitans in cultured human umbilical vein endothelial cells (HUVEC).

METHDOSCultured HUVECs were pretreated with the PAFR antagonist CV3988 or anti-human PAFR monoclonal antibody for 30 min before infection with PC-positive or -negative A. actinomycetemcomitans strains. The bacterial adhesion and invasion and cytotoxicity in the cells were examined using MTT assay.

RESULTSPretreatment with PAFR antagonists at 100, 200 and 500 nmol/L significantly reduced the adhesion rate (36.29∓3.52)%, (19.04∓3.35)% and (7.69∓3.19%), respectively] and invasion rate [(12.12∓1.58)%, (7.08∓0.29)% and (2.60∓2.26)%, respectively] of PC-positive A.actinomycetemcomitans in HUVECs. Similarly, pretreatment with anti-PAFR antibody also significantly reduced A.actinomycetemcomitans adhesion and invasion in HUVECs [(50.05∓5.28)% and (39.09∓6.50)%, respectively]. Pretreatment with PAFR antagonist (200 and 500 nmol/L) and anti-PAFR antibody (25 µg/mL) significantly increased the viability of HUVECs incubated with PC-positive A.actinomycetemcomitans from (25.39∓9.33)% to (91.12∓3.14)%, (94.12∓2.15)% and (65.5∓1.87)%, respectively, but such pretreatments did not increase the viability of cells incubated with PC-negative A.actinomycetemcomitans.

CONCLUSIONSPAFR plays an important role in the adhesion, invasion, and cytotoxicity of PC-positive A.actinomycetemcomitans in cultured HUVECs.

Aggregatibacter actinomycetemcomitans ; pathogenicity ; Bacterial Adhesion ; Cells, Cultured ; Human Umbilical Vein Endothelial Cells ; microbiology ; Humans ; Platelet Membrane Glycoproteins ; metabolism ; Receptors, G-Protein-Coupled ; metabolism

Objective To explore the splenic macrophages phenotype and secretory function of mice with periodontitis, so as to explore effects of periodontitis on macrophages. Methods 22 mice were randomly divided into periodental ligation group (group P10d ) and pseudo periodental ligation control group (group C), with 11 mice in each group. The experimental periodental ligation on mice lasted for 10 days before they were sacrificed. Flow cytometry was applied to detect the expression of M1 and M2 in mononuclear macrophages. Real-time PCR was applied to detect the relative expression of pro-inflammatory cytokines IL-1β and anti inflammatory cytokines IL-10. Results Compared with the control group C, the proportion of M1 macrophages in the periodontitis group decreased, and the ratio of M1/M2 was also decreased significantly, and IL-1β mRNA also down-regulated. Conclusions Chronic periodontal infection could down regulate the proportion of M1 macrophages , decrease ratio of M1/M2 and the expression of inflammatory cytokines IL-1βmRNA.

Objective To evaluate systematically the application effect of cluster nursing on pressure ulcer (PU) management of ICU patients.Methods Randomized control trials (RCTs) relevant to application of cluster nursing strategy in ICU PU management were collected by searching related Chinese and foreign literature published before March 2016, with the results analyzed by Stata 12. 0. Results 16 articles with 3 027 patients involved were in accordance with the criteria. It was shown in the Meta analysis that cluster nursing helped to reduce incidence of PU of ICU patients [OR=0.31,95%CI(0.25,0.38)] and to postpone occurrence of PU[WMD= 3.56, 95%CI(3.05,4.06)]. Results with all outcome indicators combined were confirmed reliable by sensitivity analysis. No existence of publication bias was shown in funnel plot. Conclusions Compared with routine nursing measures, cluster nursing showed superiority in reducing incidence rate and postponing occurrence time of PU of ICU patients, which makes it worth promoting in clinical practice.

Objective:To evaluate the effects of periodontitis on the expression of apoptosis-related proteins in pancreas of rats with Type 2 Diabetes Mellitus.Methods:Spontaneously type 2 diabetic OLETF rats were randomly divided into 2 groups:diabetes with or without periodontitis(diabetes group and combination group).LETO rats with the same germline and the same age but having normal glucose tolerance were randomly divided into control group and periodontitis group.20 weeks after periodontitis were established,all the rats were sacrificed and the pancreas were pathologically examined by HE staining.The expression of Bax,Bcl-2 and Caspase-3 in the pancreas islet were detected by immunohistochemistry staining and semi-quantitative analysis.Results:The expression of Bax, Bcl-2 and Caspase-3 in the pancreas islet was no significant difference between control and periodontitis groups(P=0.324,P=0.091,P=0.852).Compared with diabetes group,the expression of Bax and Caspase-3 in combination group showed a significant increase(P=0.000,P=0.000),and the expression of Bcl-2 was significantly decreased(P=0.022).Conclusion:Under healthy conditions,periodontitis has no effect on the expression of apoptosis-related proteins in rat pancreas islet.However,in rats with diabe-tes,periodontitis may affect the expression of apoptosis-related proteins in pancreas islet.