Aims: The main aim of this study was to screen homoeopathic medicine Acidum Muriaticum in selected potencies for its preventive and curative antimicrobial action to use for the general management of typhoid by providing individualized homoeopathic treatment for symptomatic improvement with minimal complications leading to early recovery Methodology and results: Homoeopathic medicine Acidum Muriaticum in different Hahnemannian centesimal scale potencies (CH) - (6CH, 12CH, 30CH, 200CH, 1M, 50M) was evaluated for antimicrobial activity against Salmonella Typhi (S. Typhi) (MTCC-1264) using the agar well diffusion method. The diameter of the zone of inhibition was measured (in mm) and the potency of Acidum Muriaticum having high minimum inhibitory concentration (MIC) was determined by using various controls namely, positive control (azithromycin), negative control (nutrient broth + tetracycline), vehicle control (dispensing alcohol, which contains 90% ethanol as per Homoeopathic Pharmacopoeia of India) and blank control (distilled water) in the study. Homoeopathic medicine Acidum Muriaticum in different liquid potencies (6CH, 12CH, 30CH, 200CH, 1M, 50M) was screened. Results suggest that 6CH, 12CH and 30CH potencies of Acidum Muriaticum could inhibit the growth of S. Typhi. Acidum Muriaticum showed maximum growth inhibitory zone (GIZ) against S. Typhi in agar plates with 6CH, 12CH and 30CH potencies. Acidum Muriaticum in different potencies with different concentrations significantly showed MIC against S. Typhi, namely 6CH (1:2), 12CH (1:2) in 25 µL/mL and 30CH (1:1) in 50 µL/mL concentration, respectively. Conclusion, significance and impact of study The present study concludes that the homoeopathic medicine Acidum Muriaticum in 6CH, 12CH and 30CH potencies could inhibit S. Typhi as compared with azithromycin. Hence, it could be an alternative medicine to azithromycin, the antimicrobial drug against S. Typhi. However, further research is required to confirm the precise mechanism of action.