Introduction: A bone graft is a bone replacement material that is widely used in grafting damaged bone. The bone graft material being developed is a combination of hydroxyapatite (HAp) gypsum puger and cassava starch scaffold. Bone graft material must have the characteristics of the material according to standards, osteogenesis and not cause an immunogenic response. This study aimed to analyze characteristics and responses immunogenic from a combination HAp gypsum puger scaffold cassava starch as a bone graft material. Materials and methods: Scaffold HAp gypsum puger and Cassava starch were tested using XRD, compressive test, and immunogenic assay. Application of scaffolds to rats for 7 and 14 days. Blood samples were taken and tested for IgG levels using Elisa. Results: XRD test results, obtained high peaks in HAp gypsum puger scaffold and cassava starch. compressive strength test of HAp gypsum puger scaffold and cassava starch (50:50) of 2.07 MPa. The results of Ig G on day 14 on HAp scaffolding gypsum puger and cassava starch did not cause immunogenic reactions. Conclusion: In the XRD test, high peaks are produced indicating the purity of HAp. In the compressive strength test of the HAp gypsum scaffold group Cassava starch Puger 50:50 obtained 2.07 MPa.. immunogenic test analysis of scaffold HAp gypsum puger cassava starch in rats on day 14 did not cause immunogenic reactions.