Wistar nonlinear rats weighing 170-220g. Rats were divided 5 groups, including control group, group-1, group-2, group -3 and reference group. Dried thistle extract and raw bovine testicle were contained by 1:1, 1:2 and 2:1 ratio. Each 0.1g ratio was dissolved in 20 ml distilled water and administered 2 times per day. Blood sampling was done for each rat after 3, 7, 14, 21, 28 and 35 days. Their testosterone level was measured by ELISA Kit. Results: The results indicated that free serum testosterone level in male rats increase and decrease in 7 days frequency. All tested groups showed gradual increase in the level of free serum testosterone when compared to that of corresponding control (p<0.05). Statistical comparison of all groups revealed that the maximum level was found in group 1. Moreover, group 3 was showed gradually increase in level of free serum testosterone, irrelative with period of decrease testosterone level. Conclusion: According to our results and previous study, it is suggested that preparation with Tribulus terrestris L. extract could be used in the androgen deficiency and erectile dysfunctions. Keywords: Tribulus Terrestris L, Free testosterone, Dihydrotestosterone, Protodioscin

Introduction: Pathologies of hair growth can be psychologically distressing but they are poorly controlled. Hormones and paracrine factors regulate the hair follicle and its associated glands. However, our understanding of their mechanisms is limited, restricting the development of new treatments for hair disorders. Therefore better treatments for hair loss disorders are required. Some plant extracts are believed to have effect on hair growth. Urtica Cannabina L and other are used traditionally as stimulators of human hair growth, but their effects on hair growth in vivo has not been studied yet. Goal: The aim of this study was to investigate the actual effects of those local plant extracts used as a traditional herbal treatment for hair loss, using in vivo mouse model; and to compare their effectiveness with the best medical treatment available. Material and methods: Effects of extracts from Urtica Cannabina L, and Glycyrrhiza uralensis Fisch both prepared separately and mixed at recommended concentrations. Experimental groups were compared with standard (positive control) and negative control groups. Shaved back of Balb/c mice (4 weeks old) were treated daily for 28 days (four groups, n=6 per group), and degree of their effectiveness was observed and compared with each other and with both of positive and negative control. Results: show that mixture of the two herbal extract have similar significant hair growth promotion effect compared with other groups and negative control. Therefore, extracts stimulates rodent pelage follicles in vivo, thus possible to use as promoter of hair growth. Keywords: Urtica Cannabina L, Glycyrrhiza uralensis Fisch, hair follicle, hair loss, mice.

"Introduction A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons. Distortions are frustrating but if proper corrective steps are taken peak shape distortions can be avoided. Peak purity can be established by taking the ratio of two wavelengths across the peaks of running samples or standard. May, if the peak is pure, then the ratio of wavelengths should be constant across the peak. Otherwise, the peak is impure, then the ratio between two wavelengths will change across the peak as the spectral differences caused by interfering peak change the signal ratio."

Introduction: The present study shows the processing of the pharmacopoeia article for the preparation of Di- clofenac sodium extended release matrix tablet formulated from alternative characteristic poly- mer. The matrix tablets were prepared according to traditional method of the wet granula- tion for tablet form medicine. Method: A standardization study made use of several international pharmacopoeias, such as the Mongolian National Pharmacopoeia (MNP 2011), United States Pharmacopoe- ia (USP 32), Chinese Pharmacopoeia (CP 2010) and U.S Department of F.D.A for the sub- stance’s requirements. Result: The results from this study showed a completely processed manufacturing pharma- copoeia issue of the Diclomon 100 mg retard tablet and its technological and standardized re- quirements.

Introduction In recent years we have observed that there are been more and more studies and increased reg- ulatory action regarding animal, plant and mineral-based raw materials, drugs, biological prod- ucts, groceries and food products.Therefore, dehydrated cow bile liver hydrolisate appears less harm- ful for the human body and may minimize damage to liver cells, have regenerative and healing properties, and may support the healing / recovery process process. It is important to find and apply preparations that work against acute inflammation of the liver protein, fat and carbohydrate me- tabolism. Pharmacological research was undertaken with the performance of a histomorphological assessment with reference to the hydrolisate liver, dehydrated cow bile, silymarin 3 with a composi- tion containing “Sillichol”, determining how it seriously affects the inflammation of the liver operation. Goal: To determine the presence of the liver tissue morphology with reference tothe investigational / experimental new drug “Sillichol”. Material and Methods: Male Wistar rats, specified as pathogen-free, weighing 200-250 g, were obtained from the Vivarium of the Department of Pharmacology, Drug Research Institute, and were used for the chronic CCL -induced liver injury model in all experiments. Eighteen rats were di-vided into three groups (with each group consisting of 6 rats).The rats were sacrificed at the end of the 14 days of the investigation, and the livers were im- mediately removed (Methods R.Virchow). Liver slices were made from a part of the left and cen- tral lobes, and immediately fixed in 10% buffered formalin phosphate solution, embedded in par- affin, and cut into 5μm sections. Subsequently, the sections were stained with hematoxylin and eosin (H&E) and observed under a microscope to evaluate histopathological changes (20x20). Result: Liver tissue sections of the rats were stained with H&E. The histopathological assessment in the livers was performed for all groups. Rats in the negative healthy group exhibited normal, well- defined histological structures, without any signs of vascular or inflammatory changes: no cavita- tions, necrosis or fibrosis were found in normal control sections. The histopathological analysis of the livers revealed signs of toxicity after administration of CCl .This toxicity was significant in comparison with the control group and cavitations, fibrosis in board ar- eas, mild vascular congestion and moderate inflammatory changes with congested sinusoids, nu- clear changes, and centrilobular necrosis. Sinusoids spaces were flooded with inflammatory cells. The Sillichol-treated animals of the experimental group showed a complete reversal of toxic ef- fects in the liver cells; no necrosis was seen. The central vein and portal triads appear normal and show an increase of Kupffer cells. Some of the hepatocytes indicated binucleation, suggesting re- generative activity with feathery degeneration of hepatocytes.The Carsil-treated animals of the standard group: The histology of the liver sections in rats adminis- tered with Carsil indicated significant improvement with less damage of liver tissue, as indicated by a reduced level of necrosis, narrow fibrotic septae, fat storing cells, Kupffer cells, and narrow cavita- tion. Conclusions We found that the “Sillichol” biological active product treatment reduced hepatic necrosis and fibro-cal active product improved the regeneration process of liver cells, helped to normalize cell struc- ture, and had an effect on the anti-inflammatory action in damaged liver cells. Keywords: Histology, Carbon tetrachloride, Liver damage, Silichol, Liver cell

Introduction: High-performance liquid chromatography is one of the powerful qualitative and quantitative tech-niques, generally used for the analysis of pharmaceutical preparations. HPLC’s virtue lies in its versatility. We can use it to separate compounds of molecular weights from 54-450000Daltons. Amounts of material to be detected can vary from pictograms and nanograms to micrograms and milligrams to grams depends on which kind of detectors used for chromatographically separations. This article was prepared with an aim to review different aspects of HPLC, such as principle, types, and its applications. Keywords: High performance liquid chromatography, applications, mobile phase, stationary phase

Introduction: The World Health Organization (WHO) according respiratory allergic diseases, nasal mucosal disease , which is one factor to create asthma and more than 300 million people become infected is accured in 2012[1]. In 2012, acconding to statistics of the Ministry of Health for our country was noted of 5% of the total population of the allergic disease and astma.Also In 2000, the prevalence of astma among the population was 2.4%, but in 2010 increased by 4.8%. Accordingly , the prevalence of the disease is increasing year by year, that’s why the imported new drug’s of diseases in the market and the need to produce domestically. Domestic market, we used allergic rhinitis, mucosal inflammatory treatment cholorphenamine 4 mg (Monos Industry LLC), Tavegil (Novartis Russia ), ketotifen (So pharma, Bulgaria), letizine 10 mg (KRKa, Slovani), Cetirizine 10 mg (Bertecs pharma, Russian). Letizine 10 mg, Cetirizine 10 mg drugs are contain of Ceterizine dicholoride, widely used in the treatment of foreign drug. Divided in the medical treatment of children aged 2-6 years , these drugs are expected to give a child , so we import the drug entering the appropriate doses of the company with the goal to obtain. For that purpose we determination allergon tablet’s technological process. Conclusion: Technological experiment “Alergon 5 mg ” tablets were selected ingredients of ceterizine dihydrocholoride 0.005 g, glucose monohydrate 0.09444 g, K-30 0.00006 g, magnesium stearat 0.0005 g. According to our study, ”Allergon 5 mg” tablets average weight, weigth range, disintegration, hardness, assay meets the standards set in the Mongolian National Pharmacopeia requirement of “Tablet drug ”.

Introduction: The development of a plant derived supplement with mild effects and reduced toxic and side effects for the treatment of respiratory disease is very crucial. Therefore we tested the cough suppressant effects of three plant preparations that are made from Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisch and Vaccinium Vitis idea/, plants that are common in our country. Materials and Methods:The research was conducted in 2013-2014 at the Pharmacological Laboratory of the Drug Research Institute. The experiment was carried out on four groups of 20 guinea weighing 300-400 grams and the experimental condition of coughing was generated using the method of Ucelay et al. Result:The results of the study show that the combination 3 (Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisc, Vaccinium Vitis idea) had suppressed the most when compared to the other combinations.

Introduction: Chamaenerion angustifolium (L.) Scop. has a cup-shaped nectary which locates in hollow receptacle, belonging to receptacle nectary. One layer of eqidermis on which modified stoma lie is covered by a thin cuticular layer. The nectary is differentiated from the srperfical layer cells of receptacle and that no special initial has been found. Goal: The aim of this study was to develop a natural Chamaenerion angustifolium (L.) Scop plant’s standardization requirements. Material and Methods: The root samples of natural Chamaenerion angustifolium (L.) Scop was collected from Umnudelger sum, Khentii aimag in August, 2013. The plant material dried under shade at room temperature. Then passed through 120 mesh size to remove coarse powder and fine powder was used for estimation of biologically active compound content in plant material [1, 3, 7]. Shimazdu UV –VIS Spectrophotometer was employed for all spectroscopic measurements using a pair of matched quartz cells and Shimadzu High Perpormance Liquid Chromatography equipment equipped with SPD - 20 A UV detector, CMB - 20 A system controller, CTO-10 AS vp column oven with injector and LC-6AD pumps. Gallic acid, Folin-Ciocalteau, sodium carbonate, methanol, sulfuric acid, acetic acid and glucose used were of the highest commercially available purity [8. 9, 10]. Results: The present study was carried out to develop a simple, rapid, sensitive, accurate, precise spectrophotometric and HPLC method to determine polysaccharide, polyphenolic and gallic acid by simultaneous estimation of in standardization formulation. Chromatographic analysis was carried out by Luna C18 (2) 100A reversed phase column (150 x 4.6 mm) packed with 5μm diameter particles. The mobile phase was 0.1% acedic acid:Methaol (95:5 v/v). The mobile phase was filtered through a 0.45 μm membrane filter. Then it was degassed ultrasonically prior to use. HPLC identification of standard gallic acid was at 278 nm. Flow rate and injection volume were 0.8 ml /min and 20 µl, respectively. Gallic acid was eluted with retention times of 8.2 min respectively. Amounts of gallic acid were 0.3% in plant. The standard deviation values were satisfactorily low and recovery was closed to 100% indicating the reproducibility, accuracy and precision of proposed method. The natural plant contents of the polysaccharid and polyphenolic was found 2.65% and 4.59%, respectively. Conclusion: The results from this study, we developed natural Chamaenerion angustifolium (L.) Scop. plant’s chemical ingredients for the its standardization. Keywords: Chamaenerion angustifolium (L.) Scop., polysaccharide, polyphenolic and gallic acid.

BACKGROUND: Fourteen species of the genus Iris L is grown in Mongolia. Iris L has been in traditional Mongolia medicine for treatment of urinary and kidney disease. We investigated three species of Iris L such as Iris Bunge /Iris Bungei Maxim/, Iris Tigrida /Iris Tigrida Bge / and Iris lactea /Iris Lactea Pall/ which contains rich amount of biological active substances[1,2]. Urine was collected at tubes during 4 hours, since given experimental and standard preparations orally and compared with control group. As experimental results, the urine levels of treatments groups increased 1.13 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.04 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L.\Z TOOLS AND METHODS: Adult Balb/c mice, each in the weight range of 20-28g, were obtained from the Vivarium, Drug research institute. The animals divided into five groups of four animals each and kept in cages and housed under standard conditions of temperature, humidity and dark light cycle (12h-12h). Diuretic activity was determined by the following methods of Trendelenburg et al (1980). Before experiment, all the animals received priming dose of 0.9% sodium chloride solution (0.3 ml/20g) and the treated groups were given 0.1ml/20g extracts of Iris L. The standard groups were given 0.1 ml/20g of “Nefromon. Immediately after respective treatments the animals were placed in metabolic cages and urine was collected in a measuring cylinder till 4 hours. RESULTS AND CONCLUSION: Results are shown in the ethanol extract of the whole plant of Iris Tigrida Bge at a dose of 0.1ml/20g observed diuresis during the 4 hours of the test (Tigrida Bge 1.0±0.37 ml versus control 0.55±009 ml, whereas in case of standard Nefromon the volume was found to be 0.63±0.08 ml, p<0.05). As experimental Iris L, results, urine levels of treatments groups increased 1.04 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.05 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These result, indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. KEY WORDS: Diuretic, medicinal plants, Trendelenburg methods, flavonoids