Introduction: Pathologies of hair growth can be psychologically distressing but they are poorly controlled. Hormones and paracrine factors regulate the hair follicle and its associated glands. However, our understanding of their mechanisms is limited, restricting the development of new treatments for hair disorders. Therefore better treatments for hair loss disorders are required. Some plant extracts are believed to have effect on hair growth. Urtica Cannabina L and other are used traditionally as stimulators of human hair growth, but their effects on hair growth in vivo has not been studied yet. Goal: The aim of this study was to investigate the actual effects of those local plant extracts used as a traditional herbal treatment for hair loss, using in vivo mouse model; and to compare their effectiveness with the best medical treatment available. Material and methods: Effects of extracts from Urtica Cannabina L, and Glycyrrhiza uralensis Fisch both prepared separately and mixed at recommended concentrations. Experimental groups were compared with standard (positive control) and negative control groups. Shaved back of Balb/c mice (4 weeks old) were treated daily for 28 days (four groups, n=6 per group), and degree of their effectiveness was observed and compared with each other and with both of positive and negative control. Results: show that mixture of the two herbal extract have similar significant hair growth promotion effect compared with other groups and negative control. Therefore, extracts stimulates rodent pelage follicles in vivo, thus possible to use as promoter of hair growth. Keywords: Urtica Cannabina L, Glycyrrhiza uralensis Fisch, hair follicle, hair loss, mice.

"Helicobacter pylori (H.pylori) is considered an etiologic factor for the development of peptic ulcer disease, gastric adenocarcinoma and MALT lymphoma. The combination of a proton pump inhibitor and two antibiotics has been the recommended first line therapy since the first guidelines for Helicobacter pylori infection in patients were published. However, bacterial resistance and adverse events occurrence are among most frequent causes for anti H.pylori treatment failure. More effective anti H.pylori therapy have been investigated in depth by researchers. One of the potential therapies is probiotic cultures; promising results have been observed in initial studies with numerous probiotic strains. Most of studies showed an improvement of H.pylori gastritis and decreasein H.pylori colonization after administration of probiotics. However, utilization of probiotics alone is not able to complete eradication H.pylori infection as published. The combination of some probiotic strains with standard triple therapy can reduce the occurrence of side effect due to antibiotics and increase the H.pylori eradication rate. In this article, we reviewed the possible mechanisms of action of probiotics on H.pylori and results of published studies using probiotics as possible agents to eradicate"

"Introduction A chromatographer always looks forward to getting perfect shaped peaks for each and every analysis but in reality peaks get distorted due to numerous reasons. Distortions are frustrating but if proper corrective steps are taken peak shape distortions can be avoided. Peak purity can be established by taking the ratio of two wavelengths across the peaks of running samples or standard. May, if the peak is pure, then the ratio of wavelengths should be constant across the peak. Otherwise, the peak is impure, then the ratio between two wavelengths will change across the peak as the spectral differences caused by interfering peak change the signal ratio."

Introduction In recent years we have observed that there are been more and more studies and increased reg- ulatory action regarding animal, plant and mineral-based raw materials, drugs, biological prod- ucts, groceries and food products.Therefore, dehydrated cow bile liver hydrolisate appears less harm- ful for the human body and may minimize damage to liver cells, have regenerative and healing properties, and may support the healing / recovery process process. It is important to find and apply preparations that work against acute inflammation of the liver protein, fat and carbohydrate me- tabolism. Pharmacological research was undertaken with the performance of a histomorphological assessment with reference to the hydrolisate liver, dehydrated cow bile, silymarin 3 with a composi- tion containing “Sillichol”, determining how it seriously affects the inflammation of the liver operation. Goal: To determine the presence of the liver tissue morphology with reference tothe investigational / experimental new drug “Sillichol”. Material and Methods: Male Wistar rats, specified as pathogen-free, weighing 200-250 g, were obtained from the Vivarium of the Department of Pharmacology, Drug Research Institute, and were used for the chronic CCL -induced liver injury model in all experiments. Eighteen rats were di-vided into three groups (with each group consisting of 6 rats).The rats were sacrificed at the end of the 14 days of the investigation, and the livers were im- mediately removed (Methods R.Virchow). Liver slices were made from a part of the left and cen- tral lobes, and immediately fixed in 10% buffered formalin phosphate solution, embedded in par- affin, and cut into 5μm sections. Subsequently, the sections were stained with hematoxylin and eosin (H&E) and observed under a microscope to evaluate histopathological changes (20x20). Result: Liver tissue sections of the rats were stained with H&E. The histopathological assessment in the livers was performed for all groups. Rats in the negative healthy group exhibited normal, well- defined histological structures, without any signs of vascular or inflammatory changes: no cavita- tions, necrosis or fibrosis were found in normal control sections. The histopathological analysis of the livers revealed signs of toxicity after administration of CCl .This toxicity was significant in comparison with the control group and cavitations, fibrosis in board ar- eas, mild vascular congestion and moderate inflammatory changes with congested sinusoids, nu- clear changes, and centrilobular necrosis. Sinusoids spaces were flooded with inflammatory cells. The Sillichol-treated animals of the experimental group showed a complete reversal of toxic ef- fects in the liver cells; no necrosis was seen. The central vein and portal triads appear normal and show an increase of Kupffer cells. Some of the hepatocytes indicated binucleation, suggesting re- generative activity with feathery degeneration of hepatocytes.The Carsil-treated animals of the standard group: The histology of the liver sections in rats adminis- tered with Carsil indicated significant improvement with less damage of liver tissue, as indicated by a reduced level of necrosis, narrow fibrotic septae, fat storing cells, Kupffer cells, and narrow cavita- tion. Conclusions We found that the “Sillichol” biological active product treatment reduced hepatic necrosis and fibro-cal active product improved the regeneration process of liver cells, helped to normalize cell struc- ture, and had an effect on the anti-inflammatory action in damaged liver cells. Keywords: Histology, Carbon tetrachloride, Liver damage, Silichol, Liver cell

Introduction: High-performance liquid chromatography is one of the powerful qualitative and quantitative tech-niques, generally used for the analysis of pharmaceutical preparations. HPLC’s virtue lies in its versatility. We can use it to separate compounds of molecular weights from 54-450000Daltons. Amounts of material to be detected can vary from pictograms and nanograms to micrograms and milligrams to grams depends on which kind of detectors used for chromatographically separations. This article was prepared with an aim to review different aspects of HPLC, such as principle, types, and its applications. Keywords: High performance liquid chromatography, applications, mobile phase, stationary phase

Introduction: The development of a plant derived supplement with mild effects and reduced toxic and side effects for the treatment of respiratory disease is very crucial. Therefore we tested the cough suppressant effects of three plant preparations that are made from Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisch and Vaccinium Vitis idea/, plants that are common in our country. Materials and Methods:The research was conducted in 2013-2014 at the Pharmacological Laboratory of the Drug Research Institute. The experiment was carried out on four groups of 20 guinea weighing 300-400 grams and the experimental condition of coughing was generated using the method of Ucelay et al. Result:The results of the study show that the combination 3 (Thymus gobicus Tczern, Thermopsis lanceolata L, Glycyrrhiza uralensis Fisc, Vaccinium Vitis idea) had suppressed the most when compared to the other combinations.

BACKGROUND: Fourteen species of the genus Iris L is grown in Mongolia. Iris L has been in traditional Mongolia medicine for treatment of urinary and kidney disease. We investigated three species of Iris L such as Iris Bunge /Iris Bungei Maxim/, Iris Tigrida /Iris Tigrida Bge / and Iris lactea /Iris Lactea Pall/ which contains rich amount of biological active substances[1,2]. Urine was collected at tubes during 4 hours, since given experimental and standard preparations orally and compared with control group. As experimental results, the urine levels of treatments groups increased 1.13 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.04 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L.\Z TOOLS AND METHODS: Adult Balb/c mice, each in the weight range of 20-28g, were obtained from the Vivarium, Drug research institute. The animals divided into five groups of four animals each and kept in cages and housed under standard conditions of temperature, humidity and dark light cycle (12h-12h). Diuretic activity was determined by the following methods of Trendelenburg et al (1980). Before experiment, all the animals received priming dose of 0.9% sodium chloride solution (0.3 ml/20g) and the treated groups were given 0.1ml/20g extracts of Iris L. The standard groups were given 0.1 ml/20g of “Nefromon. Immediately after respective treatments the animals were placed in metabolic cages and urine was collected in a measuring cylinder till 4 hours. RESULTS AND CONCLUSION: Results are shown in the ethanol extract of the whole plant of Iris Tigrida Bge at a dose of 0.1ml/20g observed diuresis during the 4 hours of the test (Tigrida Bge 1.0±0.37 ml versus control 0.55±009 ml, whereas in case of standard Nefromon the volume was found to be 0.63±0.08 ml, p<0.05). As experimental Iris L, results, urine levels of treatments groups increased 1.04 fold (Iris Bunge), 1.8 fold (Iris Tigrida), 1.05 fold (Iris Lacteal) and 1.15 fold (Standard or Nefromon) compared with control groups. These result, indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. These results indicated the Iris Tigrida has shown diuretic effect more than other species of Iris L. KEY WORDS: Diuretic, medicinal plants, Trendelenburg methods, flavonoids

Introduction: This plants distributed about 2500 species in worldwide and 69 species of those grow in Mongolia. In case of medicinal application of this plant is commonly used traditional medicine in European and Asian. For example, the different preparations of Astragalus mongholicus root, the most commonly used Astragalus species in Chinese medicine. Other Astragalus species which are being investigated for their anti-cancer properties are the Turkish species, and include Astragalus brachypterus, Astragalus cephalotes, Astragalus microcephalus and Astragalus trojanus. Nowdays, this plant source from nature is decreased and areal square de-escalated in Mongolia. Goals: Our main goal was conserves domesticates and improves plant genotypes of local wild medicinal specie and provides raw plant material for scientific testing from the cultivation site without depleting wild sources. Including that main goal we put a purpose of the make comparative study of vegetative organ’s anatomy morphological study on wild and planted type. Materials and Methods: The wild Astragalus mongolicus bunge was collected from Bayanchandmani sum, for Tuv province. Cropped plant was grown medicinal botanical garden of Drug research institute, Monos. Anatomy structure of vegetative organs was identified by plant anatomic method. Result: The article presents the data on structure of vegetative organs of cultivated Astragalus mongolicus Bunge and compared with wild type. Comparative studies were made on the leaf, root, stem and flower and the anatomical structure of vegetative organs of Astragalus mongolicus Bunge and wild type, which were the similar plants on botany and had the great value of medicine. The results indicated that the anatomical structures of their vegetative organs were basically the same, but there are some obvious differences on leaf and stem it depended from geographical influences. Planted plant in particular could alter their environment by affecting light and water avialablity, soil nutrient availability due to uptake. Discussion: Tserenkhand.G was studied Astragalus mongolicus root anatomy in 2003. Our root anatomy study was same to Tserenkhand.G. Conclusion: The results from this study, we are concluded that, the cultivated Astragalus mongolicus Bunge is greatly cultivated in our Botanical garden. Key words: cultivated, anatomy, epidermis