Transcriptome analysis and differential gene screening of Panax ginseng seed in different sand storage stages
10.16438/j.0513-4870.2023-0426
- VernacularTitle:不同沙藏时间人参种子转录组分析及差异基因筛选
- Author:
Guang-fei WEI
1
;
An-ning LI
2
;
Jia XU
1
;
Shi-lin CHEN
1
;
Lin-lin DONG
1
Author Information
1. Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Jilin Aodong Medicinal Seed Technology Co., Ltd., Yanbian 000623, China
- Publication Type:Research Article
- Keywords:
italic>Panax ginseng seed;
ormancy release;
transcriptome;
ifferentially expressed gene;
functional annotation
- From:
Acta Pharmaceutica Sinica
2023;58(10):3130-3139
- CountryChina
- Language:Chinese
-
Abstract:
Analyze the changes in gene expression profiles during the process of Panax ginseng seed dormancy release, and screen for differential genes, providing a basis for analyzing the mechanism of P. ginseng seed dormancy release. Comparative transcriptome analysis was conducted by using RNA-Seq sequencing technology in P. ginseng seeds stored at different low temperature. A total of 80.97 Gb of Raw reads and 80.19 Gb of Clean reads were obtained from the transcriptome. Principal component analysis and correlation analysis showed that there were significant differences in gene expression patterns at different developmental stages. Upset results showed that 46 248 unigenes were co-expressed in four stages, and 414, 445, 400 and 389 unigenes were specifically expressed in 0, 8,14 and 28 days, respectively. Gene Ontology functional annotation showed that the differentially expressed genes were mainly involved in nsaturated fatty acid biosynthetic process, nuclear body and oxidoreductase activity. Encyclopedia of Genes and Genomes metabolic pathway showed that differentially expressed genes were mainly involved in peroxisome, mitogen-activated protein kinase signaling pathway-plant, plant hormone signal transduction, ribosome, biosynthesis of unsaturated fatty acid, circadian rhythm-plant and other metabolic pathways. In the process of P. ginseng seed dormancy release, multiple biological processes, such as unsaturated fatty acid biosynthesis and plant hormone signal transduction, are required to coordinate regulation, which constitutes a complex dormancy release regulation network. Transcriptome analysis and differential gene screening of P. ginseng seeds at different sand storage time laid a foundation for the analysis of P. ginseng seed dormancy release mechanism and molecular breeding.
