Terpine-4-ol on inhibiting vascular smooth muscle cell calcification by improving mitochondrial dynamics
10.16438/j.0513-4870.2023-0219
- VernacularTitle:松油烯-4-醇通过改善线粒体动力学抑制血管平滑肌细胞钙化
- Author:
Meng-xin TU
1
;
Mei HUANG
2
;
Yan-qi ZHANG
2
;
Hong-yu CHEN
1
;
Xue-yi SHANG
1
;
Jin-jin LI
2
;
Yi-ni XU
2
;
Xiang-chun SHEN
2
;
Yan-yan ZHANG
2
Author Information
1. School of Pharmacy, Guizhou Medical University, Guiyang 550025, China; Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Guiyang 550025, China
2. Key Laboratory of Optimal Utilization of Natural Medicine Resources, Guizhou Medical University, Guiyang 550025, China
- Publication Type:Research Article
- Keywords:
terpinen-4-ol;
vascular smooth muscle cell;
osteogenic phenotype switching;
vascular calcification;
mitochondrial dynamics
- From:
Acta Pharmaceutica Sinica
2023;58(10):3024-3031
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the role and mechanism of terpinen-4-ol (T4O) on high glucose (HG) -induced calcification in vascular smooth muscle cell (VSMC). To investigate the role of T4O on HG-induced calcium deposition, osteogenic phenotypic transformation and mitochondrial dynamics in VSMC, Mdivi-1, a mitochondrial dynamin-related protein 1 (Drp-1) inhibitor, was used to analyze the correlation between mitochondrial dynamics and VSMC calcification and the role of T4O. Alizarin red S staining was used to observe calcium salt deposition and flow cytometry to detect intracellular Ca2+ content; Western blot and immunofluorescence were used to detect the expression of phenotypic switching-related markers α-smooth muscle actin (α-SMA), bone morphogenetic protein 2 (BMP2) and Runt related transcription factor 2 (Runx2), and mitochondrial dynamics-related markers mitofusin 1 (MFN1), mitofusin 2 (MFN2) and Drp-1. The results showed that low and high doses of T4O could inhibit HG-induced down-regulation of α-SMA, MFN1 and MFN2 expression levels, and up-regulation of BMP2, Runx2 and Drp-1 expression levels, reduce intracellular Ca2+ content and calcium salt deposition, and effectively inhibit HG-induced VSMC calcification and mitochondrial dynamics disorders. The T4O group, Mdivi-1 group and T4O+Mdivi-1 group were able to up-regulate the expression levels of HG-induced α-SMA, MFN1 and MFN2, down-regulate the protein expression levels of BMP2, Runx2 and Drp-1, and inhibit calcium salt deposition, and there was no significant difference between the above indexes in the T4O and T4O+Mdivi-1 groups. The above findings suggest that T4O can inhibit the expression level of Drp-1, regulate the disturbance of mitochondrial dynamics, and suppress HG-induced VSMC calcification.
