Rapid identification of chemical constituents in Xuezhikang Capsule by UPLC-Q-TOF/MSE combined with the establishment of UNIFI MS database
10.16438/j.0513-4870.2023-0008
- VernacularTitle:基于UPLC-Q-TOF/MSE的UNIFI质谱数据库建立及其在血脂康胶囊化学成分的快速分析中的应用
- Author:
Guo-fang TIAN
1
;
Lan XUE
2
;
Xi LIU
2
;
Huan PEI
2
;
Yu TANG
1
Author Information
1. Center of Pharmaceutical Technology, School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China
2. Beijing WBL Peking University Biotechnology Co., Ltd., Beijing 100083, China
- Publication Type:Research Article
- Keywords:
UPLC-Q-TOF;
MSE;
UNIFI database;
Xuezhikang Capsule;
analytical method development
- From:
Acta Pharmaceutica Sinica
2023;58(10):2862-2874
- CountryChina
- Language:Chinese
-
Abstract:
An UPLC-Q-TOF/MSE technology coupled with UNIFI database was used to develop a rapid, high coverage, accurate and efficient chemical composition qualitative method for Xuezhikang Capsule. A UNIFI database was established utilizing compound name, formula, structure, following automatic matching with high-resolution mass numbers, isotope distributions, mass deviations, fragment ion matching, and chromatographic retention features in UNIFI database to achieve the qualitative results of natural products in Xuezhikang Capsules. Combined with manual confirmation, 82 chemical components were identified in Xuezhikang Capsules, and the MS2 fragmentation pathway of typical organic acids, flavonoids, monacrines, and monascus were analyzed to ensure accuracy of the LC-MS workflow. This study clarified the chemical substance basis of Xuezhikang Capsules by LC-MS technology, providing experimental data support for the identification of key quality attributes, quality control and consistency evaluation in the manufacturing process of Xuezhikang Capsules.
