Effects of KCNQ1OT1 Gene Knockout Combined with Bruceine D on Proliferation, Migration, and Invasion of Breast Cancer MDA-MB-231 Cells
10.3971/j.issn.1000-8578.2023.23.0456
- VernacularTitle:KCNQ1OT1基因敲除联合鸦胆子素D对乳腺癌MDA-MB-231细胞增殖、迁移及侵袭的影响
- Author:
Feng LONG
1
;
Yu ZHAO
;
Yong HUANG
;
Xiaoyan LIU
;
Xuan ZHOU
;
Xue LI
;
Hailin YE
Author Information
1. School of Basic Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
Breast cancer;
Bruceine D;
KCNQ1OT1;
Migration;
Invasion
- From:
Cancer Research on Prevention and Treatment
2023;50(11):1066-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of KCNQ1OT1 gene knockout combined with bruceine D on the proliferation, migration, and invasion of breast cancer MDA-MB-231 cells. Methods Cell Counting Kit-8, wound healing, and Transwell invasion assay were used to detect the effects of bruceine D and siKCNQ1OT1 on the viability, migration, and invasion of MDA-MB-231 cells. Effect of bruceine D and siKCNQ1OT1 on the expression of KCNQ1OT1 in MDA-MB-231 cells was detected by qRT-PCR. Western blot was used to detect the effect of bruceine D and siKCNQ1OT1 on the expression of EMT-related proteins and CDC42, p-MKK7, MKK7 proteins in MDA-MB-231 cells. Results Bruceine D and siKCNQ1OT1 could significantly inhibit the viability, migration, and invasion of MDA-MB-231 cells, and the inhibitory effect was enhanced when they were combined (all P < 0.05); bruceine D downregulated the expression of KCNQ1OT1 in MDA-MB-231 cells (all P < 0.05); bruceine D combined with siKCNQ1OT1 significantly decreased CDC42, p-MKK7, N-cadherin, and Vimentin expression in MDA-MB-231 cells and increased the expression of E-cadherin (all P < 0.05). Conclusion Bruceine D combined with siKCNQ1OT1 significantly inhibit the proliferation, migration, invasion, and EMT of human breast cancer MDA-MB-231 cells, and its molecular mechanism may be related to the blocking of CDC42/MKK7 signaling pathway.
