The trend of changes in serum vitamin A, D, and E levels during pregnancy
10.3760/cma.j.cn114452-20230129-00048
- VernacularTitle:孕妇孕期血清维生素A、D和E的水平变化趋势研究
- Author:
Zhihua LYU
1
;
Juan LE
;
Wen DAI
;
Rui PENG
;
Shaoting WANG
;
Yan LI
Author Information
1. 武汉大学人民医院检验科，武汉 430060
- Keywords:
Pregnant Women;
High Performance Liquid Chromatography Tandem Mass Spectrometry;
Vitamin A;
Vitamin D;
Vitamin E
- From:
Chinese Journal of Laboratory Medicine
2023;46(8):840-844
- CountryChina
- Language:Chinese
-
Abstract:
Objective:High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to quantify the levels of vitamins A, D and E in pregnant women during the second trimester, and to investigate the change trends of serum vitamins A, D and E levels during pregnancy.Method:A total of 720 pregnant women with an average age of (29.7±4.4) years and 12-22 weeks of gestation were included from October 1, 2021 to October 30, 2022 in the obstetrics department of the People′s Hospital of Wuhan University. The concentrations of vitamins A, D and E were determined by HPLC-MS/MS. The concentration levels of each group were statistically analyzed and the deficiency rate were calculated.Results:The distribution range of vitamin A, D and E (95% CI) was 0.74-2.74 μmol/L, 2.88-25.37 ng/ml and 6.18-35.08 μmol/L, with the deficiency rates were 9.30%, 93.76% and 35.83%, respectively. Vitamin A, D and E levels in the twin group were (1.67±0.51) μmol/L, (13.18±7.44) ng/ml and 11.97 (8.85, 14.60) μmol/L, respectively. They were significantly higher than those in the singlet group (1.45±0.36) μmol/L, (10.87±5.26) ng/ml and 10.46 (6.99, 14.11) μmol/L, with statistical significance by independent sample t-test ( P<0.001). The concentration of vitamin D in the lower BMI group (<22 kg/m 2) was (12.54±5.74) ng/ml, significantly higher than that in the fat group (≥22 kg/m 2) (10.46±4.90) ng/ml, and the rank-sum test was statistically significant ( P<0.001). Conclusion:In this study, the levels of three vitamins were monitored in mid-pregnancy using HPLC-MS/MS, and the changes of serum vitamin A, D, and E levels during pregnancy were analyzed.
