Advance in the application of recombinase polymerase amplification-lateral flow strips technology in the diagnosis of pathogenic microorganisms

VernacularTitle:重组聚合酶等温扩增-侧流层析试纸条检测技术在诊断病原微生物中的应用研究进展
Author: Tuo JI ¹ ; Yuzhi GAO ; Wenjun WANG ; Chenglai XU ; Xuzhu GAO
Author Information

1. 连云港市第二人民医院（连云港市肿瘤医院）中心实验室，连云港　222000
Keywords: Clinical laboratory techniques; Noxae; Recombinase polymerase amplification
From: Chinese Journal of Laboratory Medicine 2023;46(6):634-640
CountryChina
Language:Chinese
Abstract: Recombinase polymerase amplification (RPA) is a newly developed isothermal amplification technology with high sensitivity and specificity. The combination of RPA and lateral flow strips (LFS) enables rapid identification of target genes. This technique has been widely used in medicine, food, botany, and other fields. This review generalizes the use of RPA-LFS technology for the diagnosing pathogenic microorganisms, providing a reference for point-of-care diagnosis of pathogenic microorganisms.