The effects of mechanical vibration on the expression of estrogen and brain-derived neurotrophic factor after ovariectomy
10.3760/cma.j.issn.0254-1424.2022.12.002
- VernacularTitle:机械振动对去卵巢后骨质疏松骨折大鼠雌激素和脑源性神经营养因子表达的影响
- Author:
Yijing GONG
1
;
Mingyue LIU
;
Bin LIU
;
Ting JIANG
;
Hengyi SHANGGUAN
;
Xuehong WANG
Author Information
1. 武汉体育学院，武汉 430079
- Keywords:
Osteoporosis;
Fracture;
Vibration;
Estrogen;
Neurotrophic factors
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2022;44(12):1064-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore any effect of mechanical vibration on the expression of estrogen and brain-derived neurotrophic factor (BDNF) in ovariectomized rats with an osteoporotic fracture.Methods:Thirty 3-month-old female Wistar rats were divided randomly into a control group, an ovariectomy group and a vibration group, each of 10. Fractures were induced in the rats of all three groups. Twenty minutes of whole-body vertical vibration was applied to the vibration group at a frequency of 35Hz, 5 days a week for 6 weeks. After 2 and 6 weeks the fracture healing of each group was evaluated using X-rays, the levels of hippocampal estrogen were measured using enzyme-linked immunosorbent assays and fracture-end BDNF was quantified by immunoblotting.Results:After 2 and 6 weeks of vibration the average fracture healing in the vibration group was significantly greater than in the other 2 groups. The average estrogen content in the hippocampus of the vibration group was significantly higher than in the other 2 groups after both 2 and 6 weeks, while the average BDNF content in their fracture ends was significantly lower. The BDNF expression at the fracture end was significantly correlated with the fracture healing.Conclusion:Mechanical vibration can promote the expression of estrogen and BDNF in the hippocampus and accelerate fracture healing in osteoporotic rats.
