Clustered regularly interspaced short palindromic repeats genotyping of Yersinia pestis in Yushu Tibetan Autonomous Prefecture, Qinghai Province
10.3760/cma.j.cn231583-20220521-00180
- VernacularTitle:青海省玉树藏族自治州鼠疫耶尔森菌CRISPR基因分型研究
- Author:
Juan JIN
1
;
Jian HE
;
Sheng LI
;
Xiaoyan YANG
;
Youquan XIN
;
Qi ZHANG
;
Jianguo YANG
;
Yong JIN
;
Guanghui LI
;
Xiaolu ZHANG
;
Ruixia DAI
;
Wei LI
Author Information
1. 青海省地方病预防控制所鼠疫菌专业实验室，西宁 810021
- Keywords:
Yersinia pestis;
Clustered regularly interspaced short palindromic repeats;
Genotyping
- From:
Chinese Journal of Endemiology
2023;42(2):111-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To learn about the clustered regularly interspaced short palindromic repeats (CRISPR) genotyping of Yersinia pestis in Yushu Tibetan Autonomous Prefecture (Yushu for short), Qinghai Province, and to explore its genetic characteristics. Methods:In this study, 44 representative strains isolated from local natural plague focus in Yushu from 1963 to 2007 were selected as experimental objects to extract DNA. Primers targeting the three CRISPR loci (YPa, YPb, and YPc) were designed for PCR amplification. The amplified products were sequenced and analyzed to identify the CRISPR spacer, and to determine the CRISPR genotypes and clusters.Results:Twenty-three spacers including 14 of YPa, 6 of YPb and 3 of YPc were observed among 44 strains, of which 2 spacers (a106 and a107) were firstly identified. According to the spacer arrays, the strains were divided into 15 CRISPR genotypes and classified into 6 CRISPR clusters which were Cb4, Cc3', Ca7, Ca7', CaΔ5' and Ca35', respectively. Among them, Ca7 was the most epidemic dominant cluster (34 strains) in Yushu.Conclusion:The CRISPR loci of Yersinia pestis in Yushu have multiple genotypes, high genetic polymorphism, and complex population structure.
