A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography
- Author:
Yikun LIU
1
;
Ning HE
;
Yingfang LU
;
Weiqiang LI
;
Xin HE
;
Zhentao LI
;
Zilin CHEN
Author Information
1. Department of Orthopedics Trauma and Microsurgery,Zhongnan Hospital of Wuhan University,School of Pharmaceutical Sciences,Wuhan University,Wuhan,430072,China;Key Laboratory of Combinatorial Biosynthesis and Drug Discovery,Ministry of Education,Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals,Wuhan,430071,China
- Keywords:
Sodium p-styrene sulfona;
Stationary phase;
Monolithic column;
Capillary electrochromatography;
Bifunctional interaction
- From:
Journal of Pharmaceutical Analysis
2023;13(2):209-215
- CountryChina
- Language:Chinese
-
Abstract:
Here,a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM)with reversed-phase/hydrophilic interaction liquid chromatography(RPLC/HILIC)bifunctional separation mode was success-fully prepared for capillary electrochromatography by the in situ polymerization of sodium p-styrene sulfonate(VBS)with cross-linkers 3-(acryloyloxy)-2-hydroxypropyl methacrylate(AHM)and 1,3,5-triacryloylhexahydro-1,3,5-triazine(TAT).The preparation conditions of the monolith were optimized.The morphology and formation of the poly(VBS-co-TAT-co-AHM)monolith were confirmed by scanning electron microscopy(SEM)and Fourier transform infrared spectroscopy(FT-IR).The separation perfor-mances of the monolith were evaluated systematically.It should be noted that the incorporation of VBS functional monomer can provide π-π interactions,hydrophilic interactions,and ion-exchange in-teractions.Hence,the prepared poly(VBS-co-TAT-co-AHM)monolith can achieve efficient separation of thiourea compounds,benzene series,phenol compounds,aniline compounds and sulfonamides in RPLC or HILIC separation mode.The largest theoretical plate number for N,N'-dimethylthiourea reached 1.7×105 plates/m.In addition,the poly(VBS-co-TAT-co-AHM)monolithic column showed excellent reproducibility and stability.This novel monolithic column has great application value and potential in capillary electrochromatography(CEC).
