The expression of miR-876-3p based on a rat model of bronchopulmonary dysplasia induced by hyperoxia
10.3760/cma.j.issn.1673-4912.2023.01.011
- VernacularTitle:miR-876-3p在高氧诱导新生大鼠支气管肺发育不良模型中的表达分析
- Author:
Wenxin WEI
1
;
Hua MEI
;
Chunzhi LIU
;
Yanbo ZHANG
;
Yuheng ZHANG
;
Xiaoli WANG
Author Information
1. 内蒙古医科大学附属医院新生儿科，呼和浩特 010050
- Keywords:
Bronchopulmonary dysplasia;
MiR-876-3p;
Newborn rat;
Animal model
- From:
Chinese Pediatric Emergency Medicine
2023;30(1):57-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a neonatal rat bronchopulmonary dysplasia(BPD) model induced by hyperoxia, to detect the expression of miR-876-3p in the lung tissue, and to analyze the role of miR-876-3p in the occurrence and development of BPD, so as to provide a theoretical basis for the pathogenesis, prevention and treatment of BPD.Methods:Eighty newborn SD rats were randomly divided into hyperoxia group(FiO 2 60%) and air group(FiO 2 21%). Lung tissue samples were taken on the 1st, 7th, 14th and 21st day after birth, the pathological changes of lung tissue were observed.Quantitative real-time PCR technique was used to detect the expression level of miR-876-3p. Results:Within 21 days after birth, with the prolongation of hyperoxia exposure time, the general growth of rats in hyperoxia group were lower than those in air group[14 d: (35.46±1.62) g vs.(37.08±1.25) g; 21 d: (51.92±1.83) g vs.(58.87±2.43) g]( P<0.05). On the 14th and 21st day after birth, the radial alveolar counts in lung tissue of rats in hyperoxia group were significantly reduced compared with those in air group( P<0.05). On the 7th, 14th and 21st day after birth, the alveolar septal thickness of rats in air group were lower than those in hyperoxia group( P<0.05). The expression level of miR-876-3p in hyperoxia group decreased gradually and was significantly lower on the 7th, 14th and 21st day compared with air group at the same time points[7 d: (14.97±1.13) vs.(16.64±0.89); 14 d: (11.92±0.71) vs.(16.85±0.79); 21 d: (11.39±0.79) vs.(17.52±1.17)], and the differences were all statistically significant( P all<0.01). Conclusion:In this study, a new BPD model of neonatal rats can be induced by hyperoxia and the expression level of miR-876-3p in this model is decreased.The differential expression level of miR-876-3p may play a role in the occurrence and development of BPD.
