Long non-coding RNA00461 regulates apoptosis in triple-negative breast cancer cells by combining with the proto-oncogene c-Myc
10.3760/cma.j.cn.115807-20221124-00335
- VernacularTitle:长链非编码RNA00461与原癌基因c-Myc结合调控三阴性乳腺癌细胞凋亡
- Author:
Xiulai FENG
1
;
Huifang LIU
;
Gang SUN
Author Information
1. 新疆医科大学附属肿瘤医院乳腺甲状腺外科，乌鲁木齐 830011
- Keywords:
Triple negative breast cancer;
Long non-coding RNA00461;
Apoptosis;
c-Myc
- From:
Chinese Journal of Endocrine Surgery
2023;17(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of long non-coding RNA00461 (LINC00461) on apoptosis of triple negative breast cancer cells.Methods:RT-qPCR was used to detect the expression of LINC00461 in normal breast epithelial cells MCF-10A, TNBC cell lines MDA-MB-231 and MDA-MB-468. The apoptosis rate was detected by Annexin V-FITC/PI double staining cell apoptosis kit. Western blot was used to detect apoptosis-associated protein. The localization of LINC00461 in cells was detected by FISH. RNA pulldown and RIP were used to detect the interaction between LINC00461 and c-Myc. The effect of LINC00461 on TNBC apoptosis was verified by subcutaneous tumorigenesis in nude mice.Results:Compared with MCF-10A, LINC00461 expression in MDA-MB-231 and MDA-MB-468 cells was significantly increased. Interference with LINC00461 significantly reduced the expression of LINC00461 and c-Myc protein. Overexpression of LINC00461 significantly decreased the expression level of apoptosis-related protein. Overexpression of LINC00461 significantly reduced the apoptosis rate by 50%-70%, with a statistically significant difference. FISH results showed that LINC00461 was mainly localized in the cytoplasm. RNA pulldown results showed that LINC00461 interacted with c-Myc. RIP results showed that the concentration of LINC00461 in c-Myc group was more than 400%, and the difference was statistically significant. In addition, overexpression of c-Myc reduced the promotion effect of knockdown LINC00461 on the apoptosis rate of 30%-50%. In animal experiments, overexpression of LINC00461 significantly increased tumor volume (50%-80%) and tumor weight (30%-50%) .Conclusion:LINC00461, mainly located in the cytoplasm, regulates the apoptosis of TNBC cells, and its molecular mechanism may be that LINC00461 interacts with c-Myc protein to play an important role in regulating the apoptosis of TNBC cells.
