Effect of CYFIP2 overexpression on biological function and Wnt/β-catenin signaling pathway of bladder cancer cell line T24
10.3760/cma.j.cn121382-20221001-00204
- VernacularTitle:CYFIP2过表达对膀胱癌T24细胞的生物学功能和Wnt/β-catenin信号通路的影响
- Author:
Ping XIAO
1
;
Shaohua WU
;
Xinsheng WANG
;
Hong MU
Author Information
1. 天津市第一中心医院检验科，天津 300192
- Keywords:
Cytoplasmic fragile X mental retardation protein 1 binding protein 2;
Bladder cancer;
Cell proliferation;
Cell migration;
Cell invasion;
Wnt/β-catenin sign
- From:
International Journal of Biomedical Engineering
2023;46(2):116-121
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of cytoplasmic fragile X mental retardation protein 1 binding protein 2 (CYFIP2) overexpression on the biological functions and Wnt/β-catenin signaling pathways of bladder cancer T24 cells.Methods:The control group was T24 cells transfected with the empty pcDNA3 vector, and the overexpression group was T24 cells transfected with the CYFIP2 overexpression vector. The expression of CYFIP2 mRNA and protein was detected by reverse transcriptase, quantitative polymerase chain reaction, and Western Blot. The effect of CYFIP2 overexpression on T24 cell proliferation was detected by CCK-8. The effect of CYFIP2 overexpression on T24 cell migration and invasion was detected by Transwell. The effects of CYFIP2 overexpression on Wnt/β-catenin signaling pathway in T24 cells were detected by Western Blot.Results:Compared with the control group, the expression levels of CYFIP2 mRNA and protein were increased in the overexpression group (all P < 0.001), and the cell proliferation, migration, and invasion abilities were reduced (all P < 0.01). β-catenin, c-Myc, and Cyclin D1 protein expression were down-regulated in CYFIP2 overexpressed T24 cells (all P < 0.05), while the protein levels of p-β-catenin were increased ( P < 0.05). Conclusions:CYFIP2 overexpression can inhibit T24 cell proliferation, migration, and invasion, and its possible molecular mechanism is related to the inhibition of Wnt/β-catenin signaling pathway.
