Expression of Circular RNA Hsa_circ_0026352 in Breast Cancer and Its Clinical Significance
10.3971/j.issn.1000-8578.2021.20.0310
- VernacularTitle:环状RNA Hsa_circ_0026352在乳腺癌中的表达及临床意义
- Author:
Xu ZHANG
1
;
Fang MA
;
Wei NA
;
Xiaohan LI
;
Qi HUANG
;
Jingjing YU
;
Jia WANG
;
Libin WANG
Author Information
1. Department of Beijing National Biochip Research Center Sub-center in Ningxia, General Hospital of Ningxia Medical University, Yinchuan 750004, China
- Publication Type:Research Article
- Keywords:
Circular RNA;
Breast Cancer;
Biomarker;
CircRNA microarray
- From:
Cancer Research on Prevention and Treatment
2021;48(1):43-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between the expression of Hsa_circ_0026352 and the clinical characteristics of breast cancer(BC) patients, to evaluate the value of Hsa_circ_0026352 as a diagnostic marker of breast cancer. Methods Human circRNA microarray was used to screen the different expression of circRNAs in BC tissues. qRT-PCR was used to verify the expression of Hsa_circ_0026352 in BC tissue and peripheral blood. CircRNA structure were performed by circPrimer1.2 software. T-test, ANOVA analysis, curve regression analysis and ROC curve analysis were performed to determine the diagnostic values of Hsa_circ_0026352. Results Hsa_circ_0026352 was significantly down-regulated in both breast cancer tissues and peripheral blood (P < 0.01). The AUC of Hsa_circ_0026352 were 0.881 in tissues and 0.826 in peripheral blood (both P < 0.01). The relative expression of Hsa_circ_0026352 in peripheral blood of BC cancer patients was negatively correlated with CA153 level (P < 0.05). The AUC of Hsa_circ_0026352 in peripheral blood of patients with ER positive, early stage breast cancer and breast neoplasm metastasis were 0.710, 0.771 and 0.722 (all P < 0.01), respectively. Conclusion Hsa_circ_0026352 could be a novel predictive biomarker for diagnosis of BC.
