Effects of Cyclic Strain on Neural Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells
10.16156/j.1004-7220.2023.01.22
- VernacularTitle:周期性牵张应变对大鼠骨髓间充质干细胞神经向分化的影响
- Author:
Hong CHENG
1
;
Yan HUANG
1
;
Jie YAO
1
;
Wei CHEN
1
;
Jiayi QIAN
1
;
Yubo FAN
1
Author Information
1. Beijing Advanced Innovation Center for Biomedical Engineering, Key Laboratory of Biomechanics and Mechanobiology,Ministry of Education, School of Biological Science and Medical Engineering, Beihang University
- Publication Type:Journal Article
- Keywords:
cyclic strain;
bone marrow mesenchymal stem cells (BMSCs);
neural cells;
cell differentiation
- From:
Journal of Medical Biomechanics
2023;38(1):E149-E155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanical effects of cyclic strain on neural differentiation of rat bone marrow mesenchymal stem cells (rBMSCs). Methods The rBMSCs were subjected to cyclic strain for 24 hours andthen cultured for 5 days. The expression of neural markers and the phosphorylation of relative signaling pathway proteins were evaluated. The stress distribution on cell surface was analyzed by finite element method. The differentially expressed genes induced by strain were identified by RNA sequencing analysis. Results The 0. 5 Hz strain with 5% magnitude could significantly induce higher expression of neural markers and elevated phosphorylation level of extracellular-signal-regulated kinase (ERK), protein kinase B (AKT) and mammalian target of rapamycin ( mTOR). KEGG pathway analysis showed that the focal adhesion and ECM-receptor interaction were significantly enriched under cyclic strain. Conclusions Cyclic strain could change the interaction of cells with the extracellular matrix ( ECM) and enhance the AKT/ mTOR and ERK pathway, finally promote rBMSC neural differentiation. Knowledge about the impact of mechanical stimulation on BMSC neural differentiation is expected to improve the efficiency of stem cell differentiation, shed light on device design for tissue engineering, and promote clinical application of mesenchymal stem cells in neural issue repair and regeneration.
