Effects of EW-7197 on the proliferation and migration of TGF-β1 induced human Tenon fibroblasts and its mechanism
10.3980/j.issn.1672-5123.2023.10.03
- VernacularTitle:EW-7197对TGF-β1诱导人Tenon囊成纤维细胞增殖和迁移的影响及机制
- Author:
Jia-Min WEN
1
;
Lei ZHENG
1
;
Wei-Peng AI
1
Author Information
1. Department of Pharmacy, Shenzhen University General Hospital, Shenzhen 518071, Guangdong Province, China
- Publication Type:Journal Article
- Keywords:
glaucoma;
EW-7197;
Tenon fibroblasts;
transforming growth factor-β1(TGF-β1);
scarring
- From:
International Eye Science
2023;23(10):1617-1620
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of ALK5 inhibitor EW-7197 on the proliferation and migration of human Tenon fibroblasts(HTFs)induced by transforming growth factor-β1(TGF-β1)and its mechanism.METHODS: The cell proliferation rate was detected by MTS assay, and the optimal concentration and time of EW-7197 were explored. Then HTFs were divided into three groups: normal control group, TGF-β1 induced group and TGF-β1+EW-7197 group. Cell migration was observed by Transwell assay. The protein expression levels of Fibronectin, α-SMA, as well as the phosphorylated Smad2, Smad3(p-Smad2, p-Smad3)were measured by Western blot.RESULTS: MTS assay showed that the proliferation rate of cells treated with 6.0 μmol/L EW-7197 for 24h was the lowest(all P<0.01). Transwell assay showed that the migrated number of HTFs in TGF-β1 induced group was 228.0±17.0/field, which was significantly more than that in normal control group(149.0±15.0/field)and TGF-β1+EW-7197 group(46.0±8.0/field; all P<0.01). Western blot showed that the protein relative expression levels of Fibronectin, α-SMA and p-Smad2, p-Smad3 of HTFs in TGF-β1 induced group were significantly higher than that in normal control group and TGF-β1+EW-7197 group(all P<0.001).CONCLUSION:EW-7197 can suppress the proliferation and migration of TGF-β1-induced HTFs through TGF-β/Smad signaling pathways.
