Carbohydrate-based gold nanoparticles as colorimetric sensor for cysteine
- Author:
Marc Lharen M. Barsabal
1
;
Christopher Jay T. Robidilllo
1
Author Information
- Publication Type:Journal Article
- Keywords: Gold nanoparticles; Reducing sugars
- MeSH: Cysteine; Carbohydrates; Starch
- From: Philippine Journal of Health Research and Development 2022;26(CAS Issue):68-75
- CountryPhilippines
- Language:English
-
Abstract:
Background:Gold nanoparticles have been studied extensively for their potential application in the detection of important analytes. Their relative ease of synthesis through numerous procedures makes possible their implementation in a variety of assays. Cysteine (cys), a thiol-containing amino acid implicated in numerous pathologies such as obstructive sleep apnea (OSA), has been routinely detected through expensive fluorometric assay kits.
Objectives:As such, this study aimed to develop a carbohydrate-based gold nanoparticle colorimetric assay for the convenient and straightforward detection of cys.
Methodology:Carbohydrate-based gold nanoparticles (c-AuNPs) were synthesized following a microwaveassisted procedure. The as-prepared c-AuNPs were used to detect cys by plotting the ratio of the absorbances of the aggregated and dispersed gold nanoparticles against the concentration of cys.
Results:The c-AuNP solutions were able to detect cys in the micromolar range, with the glucose-based AuNPs (glc-AuNPs) showing the widest linear range (16.7 μm to 167 μm), and the fructose-based gold nanoparticles (frc-AuNPs) exhibiting the lowest detection limit (9.0 μm) for cys. Aside from being able to detect cys, the c-AuNPs were also responsive to tyr and lys.
Conclusion:This study demonstrates that carbohydrate-based gold nanoparticles prepared following a microwave-assisted procedure using sugars as reducing agents and capping agents can be used successfully in the detection of cysteine. - Full text:571-1408-2-PB1.pdf
