Combined effect of PNPLA3 rs738409 and UGT1A1 rs10929303 gene polymorphisms on nonalcoholic fatty liver disease in children.
10.3760/cma.j.cn501113-20220222-00084
- VernacularTitle:PNPLA3 rs738409及
UGT1A1 rs10929303基因多态性对儿童非酒精性脂肪性肝病的联合作用
- Author:
Ping Ping ZHANG
1
;
Jie Yun SONG
2
;
Hai Jun WANG
1
;
Hui WANG
1
Author Information
1. Department of Maternal and Child Health, School of Public Health, Peking University, Beijing 100191, China.
2. Institute of Child and Adolescent Health, Peking University, Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
Children;
Joint effect;
Non-alcoholic fatty liver disease;
PNPLA3;
UGT1A1
- From:
Chinese Journal of Hepatology
2023;31(7):723-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the combined effects of patatin-like phospholipase domain containing 3 (PNPLA3) rs738409 (C > G) and uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) rs10929303 (C > T) on nonalcoholic fatty liver disease (NAFLD) in children and adolescents so as to provide scientific evidence for NAFLD genetic research. Methods: 1 027 children and adolescents aged 7-18 were selected as the research subjects. The general situation, past medical history, height and body weight measurements, and B- mode ultrasound test of the liver were investigated by dedicated full-time personnel. In addition, the morning fasting venous blood was collected to measure the blood biochemical indicators. DNA was extracted and genotyped for PNPLA3 rs738409 and UGT1A1 rs10929303. Logistic regression analysis was used to analyze the association and combined effect of the two gene polymorphisms and NAFLD. Statistical analysis was performed by t-test, Mann-Whitney U test, or c2 test according to different data. Results: The GG genotype of PNPLA3 rs738409 and the CC genotype of UGT1A1 rs10929303 were associated with an increased risk of developing NAFLD in children by 89% (OR = 1.89, 95% CI: 1.11-3.23, P = 0.019) and 96% (OR = 1.96, 95% CI: 1.21-3.17, P = 0.006), respectively, while the concurrent risk of NAFLD in those who carried the above two genotypes increased by 306% compared with those who did not carry both genotypes (OR = 4.06, 95% CI: 1.90 ~ 8.66, P < 0.001). Conclusion: The combined effect of PNPLA3 and UGT1A1 gene polymorphisms can significantly increase the risk of NAFLD in children, providing new evidence for elucidating the genetic susceptibility to NAFLD.
