Determination of misoprostol in workplace air by high performance liquid chromatography.
10.3760/cma.j.cn121094-20220424-00218
- VernacularTitle:工作场所空气中米索前列醇测定的高效液相色谱法
- Author:
Wei ZHAO
1
;
Shuang SONG
1
;
Hui Fang YAN
1
Author Information
1. National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
- Publication Type:Journal Article
- Keywords:
Air;
Chromatography, high performance liquid;
Misoprostol;
Workplace
- MeSH:
Chromatography, High Pressure Liquid/methods*;
Misoprostol/analysis*;
Air Pollutants, Occupational/analysis*;
Workplace;
Chromatography, Liquid
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(6):457-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a high performance liquid chromatography method for the determination of misoprostol in workplace air. Methods: From February to August 2021, the misoprostol in the workplace air was collected by glass fiber filter membrane, and theeluent was separated by C18 liquid chromatography column, determined by UV detector, and quantified by external standard method. Results: The quantitative lower limit of misoprostol determination method was 0.05 μg/ml, and the lowest quantitative concentration was 1.4 μg/m(3) (calculated by collecting 75 L air sample). The concentration of misoprostol has a good linear relationship between 0.05 to 10.00 μg/ml. The relative coefficient was 0.9998. The regression equation of the standard working curve was y=495759x-45257. The range of average recovery rates were from 95.5% to 102.8%. The intra-assay precision of the method was 1.2%-4.6%, and the inter-assay precision was 2.0%-5.9%. The samples could be stored stably for 7 days at 4 ℃. Conclusion: The high performance liquid chromatography method for the determination of misoprostol has high sensitivity, good specificity and simple procedure of sample pretreatment. It is suitable for the detection of misoprostol in the workplace air.
