Construction of a dual fluorescent reporter system for tracing horizontal transfer of mcr-1-carrying plasmid.
10.3760/cma.j.cn112150-20230103-00004
- VernacularTitle:用于追踪携带
mcr-1质粒水平转移的双荧光报告系统的构建
- Author:
Yao PENG
1
;
Lu Yan WANG
1
;
Ge ZHANG
2
;
Jia Qi LIU
2
;
Wei ZENG
3
;
Zhe LI
1
;
Xin LU
4
Author Information
1. Diarrhea Department, Institute for Communicable Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. School of Light Industry, Beijing Technology and Business University, Beijing 100048, China.
3. School of Public Health, Shandong University, Jinan 250012, China.
4. National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases/Institute for Communicable Disease Prevention and Control, Beijing 102206, China.
- Publication Type:Journal Article
- From:
Chinese Journal of Preventive Medicine
2023;57(7):1063-1067
- CountryChina
- Language:Chinese
-
Abstract:
The green fluorescent reporter gene was inserted into the gene interval of polymyxin resistant mcr-1-carrying plasmid (pSH13G841) by homologous recombination of suicide plasmid. At the same time, E. coli J53 with red fluorescent reporter gene was constructed. Using the ability of spontaneous conjugation of drug resistant plasmid (pSH13G841), pSH13G841-GFP plasmid was transferred into J53 RFP bacteria to construct a double fluorescent labeled donor bacterium. The two light-emitting systems could stably and spontaneously express fluorescence without mutual interference. The dual fluorescence report system constructed can be used for visual tracing horizontal transfer of mcr-1-carrying plasmid, the subsequent model can study the colonization, transfer and prognosis of drug-resistant bacteria/drug-resistant genes mcr-1 by using mouse in vivo imaging technology.
