Analysis and Application of Seven Common New Psychoactive Substances of Synthetic Cannabinoids in Hair.
10.12116/j.issn.1004-5619.2021.310301
- Author:
Yan SHI
1
;
Li Ying ZHOU
1
;
Bao Hua SHEN
1
;
Hang CHEN
1
;
Meng Xi LIU
1
;
Ping XIANG
1
;
Wei LIU
1
Author Information
1. Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
- Publication Type:Journal Article
- Keywords:
forensic medicine;
toxicological analysis;
ultra-high performance liquid chromatography（UPLC）;
tandem mass spectrometry;
new psychoactive substance;
synthetic cannabinoid;
4F-MDMB-BUTINACA;
hair
- MeSH:
Cannabinoids;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Hair;
Tandem Mass Spectrometry
- From:
Journal of Forensic Medicine
2021;37(4):479-485
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a detection method for common new psychoactive substances of synthetic cannabinoids in hair with ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods In the 1 mL of internal standard methanol solution, 20 mg hair was added. After cryogenic grinding and ultrasonic extraction, the extract was separated by ACQUITY UPLC HSS T3 column （100 mm×2.1 mm, 1.8 μm）. The mobile phase A was aqueous solution that composed of 20 mmol/L ammonium acetate, 0.1% formic acid, and 5% acetonitrile. The mobile phase B was acetonitrile. Electrospray ionization source in positive ion mode was used for data acquisition in multi-reaction monitoring （MRM） mode. Results The seven common new psychoactive substances of synthetic cannabinoids in hair had a good linear relationship within their respective linear ranges （r>0.99）, the limits of detection were 0.5-2 pg/mg, the limits of quantification were 1-5 pg/mg, the intra-day and inter-day precisions were 0.1%-12.6%, the intra-day and inter-day accuracies were 89.2%-110.7%, the recovery rates were 52.3%-93.3%, and the matrix effects were 19.1%-95.2%. Conclusion The established method has a simple sample preparation process and high sensitivity. It is suitable for qualitative and quantitative analysis of common new psychoactive substances of synthetic cannabinoids in hair.
