Genetic Structure Analysis of Y Chromosome STR and SNP in Population of Changshu.
10.12116/j.issn.1004-5619.2020.04.020
- Author:
Xiao TANG
1
;
Jun Zhe TIAN
1
;
Yi Wei ZHANG
1
;
Cai Yong YIN
1
;
Zhen TANG
1
;
Shuai LI
1
;
Jun YAO
1
;
Jia Wei HUANG
2
;
Shi Lin LI
1
Author Information
1. School of Life Sciences, Fudan University, Shanghai 200438, China.
2. Changshu Public Security Bureau, Changshu 215500, Jiangsu Province, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
short tandem repeat;
single nucleotide polymorphism;
Y chromosome;
genetic structure;
haplogroup;
Han nationality;
Changshu
- MeSH:
Chromosomes, Human, Y/genetics*;
Gene Frequency;
Genetics, Population;
Haplotypes;
Humans;
Male;
Microsatellite Repeats;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2020;36(4):538-544
- CountryChina
- Language:English
-
Abstract:
Objective To provide a theoretical basis for building a Y chromosome database in specific regions by analyzing the pedigree specific core haplogroup and region specific genetic structure in Changshu. Methods One thousand seven hundred and two samples from unrelated Han male individuals in Changshu were collected. Then 27 Y-STR were genotyped through YfilerTM Plus PCR Amplification Kit, Y-SNP haplogroup of each sample was speculated using Y-Predictor software and some samples were verified by amplification refractory mutation system-polymerase chain reaction （ARMS-PCR）. Results A total of 1 556 haplotypes were found on the 27 Y-STR genetic markers of the 1 702 samples. The haplotype diversity （HD） value was 0.999 827. DYS385 （0.933） had the highest gene diversity （GD） value while DYS438 （0.409） had the lowest. By the Y-Predictor software, all samples were confirmed to be from 162 sub-haplogroups of C, D, N, O, Q and R. Samples were randomly selected to verify the prediction results by the software and the prediction accuracy of Y-Predictor software was as high as 95.74%. Conclusion This study found that 27 Y-STR genetic markers have relatively high polymorphisms in the Changshu population, and have good forensic individual identification and paternity testing ability.
