Genetic Polymorphism and Forensic Application of 47 Autosomal InDel Loci in 5 Chinese Ethnic Groups.
10.12116/j.issn.1004-5619.2020.04.019
- Author:
Xi Yong PAN
1
;
Chang Hui LIU
2
;
Wei An DU
3
;
Ling CHEN
4
;
Xiao Long HAN
2
;
Xing Yi YANG
2
;
Yue LI
2
;
Chao LIU
2
Author Information
1. Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
2. Guangzhou Criminal Science and Technology Institute, Guangzhou 510030, China.
3. AGCU ScienTech Incorporation, Wuxi 214174, Jiangsu Province, China.
4. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
forensic genetics;
polymorphism, genetic;
insertion/deletion;
individual identification;
Guangdong;
Guangxi
- MeSH:
Asian People/genetics*;
China;
Ethnicity/genetics*;
Female;
Gene Frequency;
Genetic Loci;
Genetics, Population;
Humans;
INDEL Mutation;
Male;
Microsatellite Repeats;
Polymorphism, Genetic
- From:
Journal of Forensic Medicine
2020;36(4):531-537
- CountryChina
- Language:English
-
Abstract:
Objective To investigate the population genetic data of 47 autosomal insertion/deletion （InDel） polymorphism genetic markers involved in AGCU InDel 50 kit in Guangdong Han, Guangxi Zhuang, Guangxi Yao, Guangxi Jing, and Guangxi Mulam, and to evaluate their application in forensic DNA identification. Methods Multiplex amplification of the 768 unrelated individuals from the 5 ethnic groups mentioned above was performed with the AGCU InDel 50 kit. Genotyping was carried out by 3500xL gene analyzer, population genetic parameters were gathered and polymorphism analysis was performed. Results No linkage disequilibrium was found among 47 autosomal InDel loci in the 5 ethnic groups. The distribution of genotype frequency of 47 autosomal InDel loci confirmed to the Hardy-Weinberg equilibrium in Guangdong Han and Guangxi Zhuang. Except for rs139934789, the other 46 loci confirmed to the Hardy-Weinberg equilibrium in Guangxi Yao, Guangxi Jing, and Guangxi Mulam. The results of genetic variation analysis among the populations showed that 1.12% of genetic variation was caused by ethnic group differences. The cumulative discrimination power of 47 autosomal InDel loci for the 5 ethnic groups were all above 0.999 999 999 999 999. The cumulative probability of exclusion for each ethnic group was less than 0.999 9. The two Y-InDels were identified in all male individuals and were absent in all female individuals. Conclusion Except for rs139934789, the other 46 InDel loci have a relatively good genetic polymorphism in the 5 Chinese ethnic groups, and can be used for forensic individual identification and as effective supplements for paternity testing.
