Application of Quantitative Analysis of Diatoms in Lung Tissue for the Diagnosis of Drowning of Experimental Animals.
10.12116/j.issn.1004-5619.2019.06.011
- Author:
Jian ZHAO
1
;
Yin Zhou LUO
2
;
Yu Zhong WANG
3
;
Cheng XIAO
4
;
He SHI
5
;
Sun Lin HU
5
;
Xiao Dong KANG
5
;
Qu Yi XU
5
;
Jian Ding CHENG
1
;
Chao LIU
1
Author Information
1. Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
2. Shiyan Public Security Bureau, Shiyan 442000, Hubei Province, China.
3. Guangdong Jiangmen Chinese Medical College, Jiangmen 529000, Guangdong Province, China.
4. School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
5. Key Laboratory of Forensic Pathology, Institute of Guangzhou Forensic Science, Ministry of Public Security, PRC, Guangzhou 510030, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
drowning;
diatoms;
animals, laboratory;
rabbits;
swine;
lung
- MeSH:
Animals;
Autopsy;
Diatoms/cytology*;
Drowning/diagnosis*;
Lung;
Microscopy, Electron, Scanning;
Rabbits;
Random Allocation;
Swine
- From:
Journal of Forensic Medicine
2019;35(6):706-709
- CountryChina
- Language:English
-
Abstract:
Objective To discuss the application value of diatom examination in lung tissue for the forensic diagnosis of drowning. Methods The experimental animals were divided randomly into drowning, postmortem submergence and dying on land group. Diatoms in lung tissue and drowning fluid were analyzed quantitatively by microwave digestion-vacuum filtration-automated scanning electron microscopy diatom examination method. The ratios of content of diatoms in lung tissue and drowning fluid （CL/CD ratio） were recorded. Results The CL/CD ratios of experimental rabbits in the drowning group （5.82±3.50） were much higher than that of postmortem submergence group （0.47±0.35）; the CL/CD ratios of different parts of the lung lobes of experimental pigs in the drowning group were higher than that of postmortem submergence group （P<0.05）; in seawater, brackish water, river fresh water and lake fresh water, the CL/CD ratios of experimental pigs in the drowning group were higher than that of postmortem submergence group （P<0.05）. In animal experiments, all the cases with CL/CD ratio >1.6 were from drowning group. Conclusion CL/CD ratio is an indicator with good application prospects in the diagnosis of drowning.
