Difference Analysis Based on 16S rRNA Sequencing of Different Soil Bacterial Communities.
10.12116/j.issn.1004-5619.2019.02.011
- Author:
Guo Qing SONG
1
;
Hui LI
2
;
Ke MA
2
;
Xue Ying ZHAO
2
;
Yi Wen SHEN
1
;
Jian Hui XIE
1
;
Huai Gu ZHOU
1
Author Information
1. Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
2. Shanghai Research Institute of Criminal Science and Technology, Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
- Publication Type:Journal Article
- Keywords:
forensic pathology;
forensic genetics;
soil microbiology;
DNA, bacterial;
RNA, ribosomal, 16S;
biodiversity;
high-throughput sequencing
- MeSH:
Biodiversity;
China;
DNA, Bacterial/genetics*;
Forensic Genetics;
High-Throughput Nucleotide Sequencing/methods*;
Phylogeny;
RNA, Ribosomal, 16S/genetics*;
Soil Microbiology
- From:
Journal of Forensic Medicine
2019;35(2):187-193
- CountryChina
- Language:English
-
Abstract:
Objective To study the structure and differences of bacterial communities in different soils, and to explore the effectiveness of 16S rRNA sequencing in identification of different soil. Methods Soil samples from 7 places in Shanghai were collected, then bacterial genomic DNA were extracted from them. The fragments of hypervariable region from 16S rRNA sequences were sequenced with high-throughput sequencing techniques. The results were quantified or visualized with bioinformatics software. The differences in diversity and abundance among the three kinds of bacterial communities in soil samples from grassland, forests and beaches were compared and analyzed. Results The statistical differences that existed among the alpha diversity indexes of bacterial communities in soil samples of grassland, forests and beaches had statistical significance. The relative abundance and diversity of bacterial communities in these three kinds of soil were significantly different. Grassland soil had higher Acidobacteria abundance, forest soil had higher Proteobacteria abundance, beach soil had higher Actinobacteria abundance. However, the differences in soil bacterial communities in artificial grasslands, natural grasslands and industrial district grasslands did not have statistical significance. Conclusion 16S rRNA sequencing can effectively distinguish different soils. This method may be able to provide clues for first crime scene inference in criminal cases.
