Genes Expression in the Early Stage of Acute Renal Ischemia-reperfusion Injury in Rats.
10.3969/j.issn.1004-5619.2016.06.001
- Author:
Jun Yi LIN
1
;
Xing MAO
2
;
Hui Juan WU
2
;
Ai Ming XUE
1
Author Information
1. Department of Forensic Medicine, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
2. Department of Pathology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
- Publication Type:Journal Article
- Keywords:
bioinformatics;
differential gene expression;
forensic pathology;
reperfusion injury
- MeSH:
Acute Kidney Injury/genetics*;
Animals;
Annexin A1/genetics*;
Apoptosis Regulatory Proteins/genetics*;
Gene Expression;
Kidney/pathology*;
Kruppel-Like Factor 6/genetics*;
Rats;
Real-Time Polymerase Chain Reaction;
Reperfusion Injury/genetics*;
Signal Transduction
- From:
Journal of Forensic Medicine
2016;32(6):401-405
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the differential genes expression in the early stage of acute renal ischemia-reperfusion injury and explore potential molecular mechanisms.
METHODS:The ischemia-reperfusion model was made via clamping renal artery of rat. The microarray detection and bioinformatics analyzing of the genes expression were performed. Differentially expressed genes were screened and related cellular activities and signaling pathways were analyzed in early stage of acute kidney injury. Meanwhile, molecules closely relative to acute kidney injury were explored by establishing a biological network of the differentially expressed genes, and the results were verified by real-time PCR.
RESULTS:A total of 151 genes showed differential expression in this study, including 132 up-regulated and 19 down-regulated genes. Cell proliferation, cytokines mediated signaling transduction and immune responses were greatly enriched by GO and KEGG analysis. The results of real-time PCR showed that compared with control groups, three selected genes （ANXA1, PHLDA1 and KLF6） which related to the acute kidney injury had an obvious differential expression in the early stage of disease. The multiple of increase was essentially the same as the multiple detected by microarray.
CONCLUSIONS:This study shows differential gene expression profile, related biological processes and signaling pathways involved in the early stage of acute kidney injury. ANXA1, PHLDA1 and KLF6 may play a role in the pathogenesis of acute kidney injury.
