Isolation and culture of neural stem cells in ventral midbrain of a rat embryo
- VernacularTitle:大鼠胎鼠中脑神经干细胞的分离培养
- Author:
Xuekun LI
;
Anchen GUO
;
Pingping ZUO
- Publication Type:Journal Article
- Keywords:
ventral midbrain of rat embryo, neural stem cell, isolation, culture
- From:
Chinese Journal of Rehabilitation Theory and Practice
2003;9(7):394-396
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a method of isolation and culture of neural stem cells(NSCs). MethodsTissues of ventral midbrain were isolated from a rat embryo,and the NSCs were cultured stimulated with basic fibroblast growth factor(bFGF)in vitro.The cells were identified by immunocytochemistry.ResultsNSCs proliferated into neurosphere in symmetric and non-symmetric cleavage ways,and differentiated into neuron, astroglia and oligodendrosyte. ConclusionsThe method has been established to isolate and culture the neural stem cells.
