Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media.
- Author:
Hao-yue ZHANG
;
Su-jun XU
;
Xiao-xian TANG
;
Ji-jun NIU
;
Xiang-jie GUO
;
Cai-rong GAO
- Publication Type:Journal Article
- MeSH:
Anaphylaxis/diagnosis*;
Basophils/cytology*;
Carboxypeptidases A/metabolism*;
Complement Membrane Attack Complex/metabolism*;
Contrast Media;
Flow Cytometry;
Granulocytes/cytology*;
Humans;
Mast Cells/cytology*;
Tetraspanin 30/metabolism*
- From:
Journal of Forensic Medicine
2015;31(3):188-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the diagnostic significance of basophil activation test (BAT) in anaphylaxis to non-ionic contrast media through testing the content of CD63, mast cell-carboxypeptidase A3 (MC-CPA3), and terminal complement complex SC5b-9 of the individuals by testing their levels in the normal immune group and the anaphylaxis groups to β-lactam drugs and non -ionic contrast media.
METHODS:The CD63 expression of basophilic granulocyte in blood was detected by flow cytometry. The levels of MC-CPA3 in blood serum and SC5b-9 in blood plasma were detected by ELISA.
RESULTS:The CD63 expression of basophilic granulocyte in blood, the levels of MC-CPA3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs were significantly higher than that in normal immune group (P < 0.05).
CONCLUSION:There is activation of basophilic granulocytes, mast cells and complement system in anaphylaxis to non-ionic contrast media. BAT can be used to diagnose the anaphylaxis to non-ionic contrast media.
