Chlorpyrifos Determined in Human Blood by UPLC-MS/MS and Its Application in Poisoning Cases.
- Author:
Zheng QIAO
;
Hui YAN
;
Xian-yi ZHUO
;
Bao-hua SHEN
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Chlorpyrifos/blood*;
Chromatography, High Pressure Liquid/methods*;
Chromatography, Liquid/methods*;
Humans;
Limit of Detection;
Poisoning;
Reproducibility of Results;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2015;31(2):112-116
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To determine the chlorpyrifos in human blood by liquid chromatography-tandem mass spectrometry and to validate its application in poisoning cases.
METHODS:The samples were extracted by a simple one-step protein precipitation procedure. Chromatography was performed on a Capcell Pack C18 MGII column (250 mm x 2.0 mm, 5 μm) using an isocratic elution of solvent A (0.1% formic acid-water with 2 mmol/L ammonium acetate) and solvent B (methanol with 2 mmol/L ammonium acetate) at 5:95 V:V).
RESULTS:The linear ranged from 5 to 500 ng/mL (r = 0.998 7). The limit of detection (LOD) and the lower limit of quantification (LLOQ) were 2 ng/mL and 4 ng/mL, respectively. For this method, the precision and accuracy of intra-day and inter-day were < 10% and 97.44%-101.10%, respectively. The results in stability test of long-term frozen were satisfied. The matrix effect, recovery and process efficiency were 64.97%-86.81%, 76.70%-85.52%, and 55.57%-66.58%, respectively.
CONCLUSION:This method can provide a rapid approach to chlorpyrifos extraction and determination in toxicological analysis of forensic and clinical treatment.
