The Control of Drug Release and Vascular Endothelialization after Hyaluronic Acid-Coated Paclitaxel Multi-Layer Coating Stent Implantation in Porcine Coronary Restenosis Model.
- Author:
In Ho BAE
1
;
Myung Ho JEONG
;
Ju Han KIM
;
Yong Hwan PARK
;
Kyung Seob LIM
;
Dae Sung PARK
;
Jae Won SHIM
;
Jung Ha KIM
;
Youngkeun AHN
;
Young Joon HONG
;
Doo Sun SIM
Author Information
- Publication Type:Original Article
- Keywords: Stents; Hyaluronic acid; Paclitaxel; Preclinical drug evaluation; Coronary restenosis
- MeSH: Animal Experimentation; Coronary Restenosis*; Coronary Vessels; Drug Evaluation, Preclinical; Drug Liberation*; Drug-Eluting Stents; Extracellular Matrix; Hyaluronic Acid; In Vitro Techniques; Myocytes, Smooth Muscle; Paclitaxel*; Polymers; Stents*
- From:Korean Circulation Journal 2017;47(1):123-131
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND AND OBJECTIVES: Hyaluronic acid (HA) is highly biocompatible with cells and the extracellular matrix. In contrast to degradation products of a synthetic polymer, degradation products of HA do not acidify the local environment. The aim of this study was to fabricate an HA-coated paclitaxel (PTX)-eluting stent via simple ionic interactions and to evaluate its effects in vitro and in vivo. MATERIALS AND METHODS: HA and catechol were conjugated by means of an activation agent, and then the stent was immersed in this solution (resulting in a HA-coated stent). After that, PTX was immobilized on the HA-coated stent (resulting in a hyaluronic acid-coated paclitaxel-eluting stent [H-PTX stent]). Study groups were divided into 4 groups: bare metal stent (BMS), HA, H-PTX, and poly (L-lactide)-coated paclitaxel-eluting stent (P-PTX). Stents were randomly implanted in a porcine coronary artery. After 4 weeks, vessels surrounding the stents were isolated and subjected to various analyses. RESULTS: Smoothness of the surface was maintained after expansion of the stent. In contrast to a previous study on a PTX-eluting stent, in this study, the PTX was effectively released up to 14 days (a half amount of PTX in 4 days). The proliferation of smooth muscle cells was successfully inhibited (by 80.5±12.11% at 7 days of culture as compared to the control) by PTX released from the stent. Animal experiments showed that the H-PTX stent does not induce an obvious inflammatory response. Nevertheless, restenosis was clearly decreased in the H-PTX stent group (9.8±3.25%) compared to the bare-metal stent group (29.7±8.11%). CONCLUSION: A stent was stably coated with PTX via simple ionic interactions with HA. Restenosis was decreased in the H-PTX group. These results suggest that HA, a natural polymer, is suitable for fabrication of drug-eluting stents (without inflammation) as an alternative to a synthetic polymer.
