Simultaneous determination of sixteen antibiotics in human urine with ultra performance liquid chromatography-tandem mass spectrometry.
- Author:
Cong CHEN
1
;
Hui YAN
;
Bao-hua SHEN
;
Xian-yi ZHUO
Author Information
1. Department of Forensic Medicine, Shanghai Medical College of Fudan University, Shanghai 200032, China.
- Publication Type:Journal Article
- MeSH:
Ampicillin/urine*;
Anti-Bacterial Agents/urine*;
Chromatography, High Pressure Liquid/methods*;
Forensic Toxicology;
Humans;
Hydrogen-Ion Concentration;
Penicillins/urine*;
Reproducibility of Results;
Sensitivity and Specificity;
Solid Phase Extraction;
Solvents/chemistry*;
Spectrometry, Mass, Electrospray Ionization/methods*;
Tandem Mass Spectrometry/methods*;
Time Factors
- From:
Journal of Forensic Medicine
2011;27(1):25-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for simultaneous determination of sixteen antibiotics in human urine by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).
METHODS:With Piperacillin as an internal standard, the target antibiotics in urine samples were enriched and purified by Oasis HLB solid phase extraction (SPE) cartridges, then separated in a ZORBAX SB-C18 column with a gradient elution of mobile phase of 0.1% formic acid water and acetonitrile, finally analyzed with multiple reaction monitoring (MRM) mode.
RESULTS:The limits of detection (LOD) for these sixteen antibiotics were in the range of 0.05-10.0 ng/mL and the limits of quantification (LOQ) in the range of 0.25-20.0 ng/mL. Within the related linear range, the related coefficient (r) of sixteen antibiotics were all more than 0.995. Accuracies for these antibiotics were ranged from 82.0%-119.3%, the within-day precision were less than 13.9%.
CONCLUSION:The developed method is sensitive, specific and appropriate for the analysis of antibiotics in forensic toxicology and therapeutic drugs monitoring.
