Simultaneous determination of opioid compounds in human urine by UPLC-MS/MS.
- Author:
Chang-Shu ZHAN
1
;
Bao-Hua SHEN
;
Xian-Yi ZHUO
Author Information
1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. zcs5126@yahoo.com.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Chromatography, Liquid/methods*;
Codeine/urine*;
Humans;
Limit of Detection;
Morphine/urine*;
Morphine Derivatives/urine*;
Reproducibility of Results;
Sensitivity and Specificity;
Substance Abuse Detection/methods*;
Tandem Mass Spectrometry/methods*
- From:
Journal of Forensic Medicine
2010;26(3):188-191
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To propose a method for simultaneous determination of codeine(COD), 6-monoacetyl-morphine (6-MAM), morphine (MOR), morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G) in human urine by ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS).
METHODS:After precipitation of protein by acetonitrile, the urine samples, with added the morphine-d3 (MOR-d3) and morphine-3-Glucuronide-d3 (M3G-d3) as internal standards, were pre-treated by Sirocco protein precipitation plate, and then analyzed by UPLC-MS/MS.
RESULTS:The limit of detection was 0.2 ng/mL for both COD and MAM, the limit of quantitation was 0.5 ng/mL for both COD and MAM. The limit of detection was 0.5 ng/mL for MOR, M3G and M6G, the limit of quantitation was 1 ng/mL for them. The linear correlation coefficients were not less than 0.9997, both the inter-day and intra-day precisions were less than 10%, the recoveries were in the range of 70.0% to 98.3%, the matrix effects were about 50.5% to 99.0%.
CONCLUSION:This proposed method is simple, rapid and accurate, it could be applied in forensic toxicological analysis.
