X-sNP genotyping using the TaqMan probe technology.
- Author:
Su-hua ZHANG
1
,
2
;
Li LI
;
Cheng-tao LI
;
Shu-min ZHAO
Author Information
1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China. zsh-daisy@
2. com
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Alleles;
Asian People/genetics*;
China/ethnology*;
Chromosomes, Human, X/genetics*;
DNA/genetics*;
DNA Probes;
Female;
Fluorescent Dyes;
Forensic Genetics;
Gene Frequency;
Genotype;
Humans;
Male;
Polymerase Chain Reaction/methods*;
Polymorphism, Single Nucleotide/genetics*;
Sensitivity and Specificity;
Taq Polymerase
- From:
Journal of Forensic Medicine
2010;26(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a rapid, accurate and economical real time fluorescence PCR method with TaqMan probe technology to detect the X chromosome single nucleotide polymorphism (X-SNP).
METHODS:TaqMan probes and polymerase chain reaction primers were respectively designed according to the 13 X-SNP. Then, the X-SNP were genotyped after the amplification by real time fluorescence PCR.
RESULTS:All the loci follow the Hardy-Weinberg equilibrium. The polymorphic information content for 13 distinct loci varied between 0.3497 and 0.3750 while the heterozygosity ranged from 0.4537 to 0.5021. A real time fluorescent PCR method based on TaqMan probe was successfully developed and the results were accordant with those analyzed by DNA sequencing of the 13 X-SNP.
CONCLUSION:The allele specific real time fluorescence PCR based on TaqMan probe is a sensitive, simple technology and suitable for rapid analysis of XSNP. All the loci show highly polymorphic and may be potential in forensic genetics.
