Analysis of multiple cephalosporins in blood and urine by HPLC.
- Author:
Mu LI
1
,
2
;
Lin-Chuan LIAO
;
You-Yi YAN
;
Lin YANG
;
Xiao-Min LIU
;
Jin DAI
;
Xiao-Na MA
;
Xin SHENG
;
Hong HUANG
;
Gui SU
Author Information
1. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. moonlee125@
2. com
- Publication Type:Journal Article
- MeSH:
Adult;
Anti-Bacterial Agents/urine*;
Cefazolin/urine*;
Cefoperazone/urine*;
Cefotaxime/urine*;
Cephalosporins/urine*;
Cephradine/urine*;
Chromatography, High Pressure Liquid/methods*;
Forensic Toxicology;
Humans;
Male;
Sensitivity and Specificity;
Specimen Handling
- From:
Journal of Forensic Medicine
2009;25(6):437-439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a new high performance liquid chromatography (HPLC) method for determining the concentration of cefazolin, cefradine, cefoperazone and cefotaxime in blood and urine, as well as to investigate its applicability.
METHODS:Protein in blood and urine was precipitated directly by acetonitrile with acetanilide was used as the internal standard using Agilent Zorbax SB-Aq column (250 mm x 4.6 mm, 5 microm). The mixed solvents of water (triethylamine 0.12%, acetic acid 0.12%) and acetonitrile were used as the mobile phase to separate cephalosporins using gradient elution method at 1 mL/min (flow rate) and 254 nm (detection wavelength).
RESULTS:The working curve of four cephalosporins showed a good correlation (r = 0.9993), with the detection limit up to 0.01 microg/mL. The recovery rate was more than 81.2%.
CONCLUSION:This method is fast, easy and accurate. It is suitable for biological analysis of the 4 cephalosporins of the blood and urine in practical cases.
