The changes of Fourier transform infrared spectroscopy in rat's brain.
- Author:
Yong KE
1
;
Jian-Gang ZHANG
;
Ping HUANG
;
Qing-Yang LU
;
Shuan-Liang FAN
;
Yong-Cheng XU
;
Zhen-Yuan WANG
Author Information
1. Department of Forensic Medicine, Medical College, Xi'an Jiaotong University, Xi'an 710061, China. keyong_best@stu.xjtu.edu.cn
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Animals;
Brain Chemistry;
Death;
Forensic Pathology/methods*;
Male;
Postmortem Changes;
Rats;
Rats, Sprague-Dawley;
Spectroscopy, Fourier Transform Infrared;
Time Factors
- From:
Journal of Forensic Medicine
2008;24(3):161-164
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To apply Fourier transform infrared (FTIR) spectroscopy to study the process of postmortem degradation of the rat brain and to provide a new way for the estimation of postmortem interval (PMI).
METHODS:The rats were sacrificed by cervical dislocation and the bodies were kept in a controlled environmental chamber set at (30 +/- 2) degrees C. To measure the content of the chemical groups in postmortem rat brains at the different time points from 0 to 36 h using the FTIR spectrograph.
RESULTS:With prolongation of PMI, the peak position of main absorbance bands in the FTIR spectra showed no significant changes, while the peak levels showed dramatic changes: (1) The relative peak intensity of 1080 cm(-1), 1238 cm(-1) (I1080/I1398, I1238/I1398) associated with nucleic acid decreased obviously; (2) The peak intensity ratio at Amide I, II (I1647/I1541) decreased; (3) The peak intensities at 1456 cm(-1) and 1398 cm(-1) showed a decreased and an increased trend, respectively; (4) Compared to the peak intensity of 1647 cm(-1), the peak intensities at 2852 cm(-1), 2871 cm(-1), 2923 cm(-1), and 2958 cm(-1) tended to increase, with only a slightly increased tendency in peak intensity of 2871 cm(-1).
CONCLUSION:FTIR spectroscopy may be potentially used as an effective method for estimating the PMI in medicolegal practice using brain tissue sample.
