Study of Papaver somniferum cultivars identification by TD-RAPD technique.
- Author:
Fan LU
1
;
Jun-Yan HONG
;
Rui HE
;
Li-Sha LI
Author Information
1. Yunnan Criminal Science and Technology Institute, Kunming 650021, China.
- Publication Type:Journal Article
- MeSH:
DNA Primers;
DNA, Plant/isolation & purification*;
Feasibility Studies;
Humans;
Papaver/genetics*;
Plant Leaves/genetics*;
Polymerase Chain Reaction;
Random Amplified Polymorphic DNA Technique/methods*;
Species Specificity
- From:
Journal of Forensic Medicine
2006;22(5):367-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:The feasibility of Papaver somniferum L. cultivars identification was explored by TD-RAPD technique.
METHOD:Genomic DNA was extracted by improved CTAB method. One sample of species from Papaver somniferum L in xishuangbanna area. was studied by using TD-RAPD method.
RESULT:We established an optimal method of extracting genomic DNA. Six primers were picked out from 10 primers.
CONCLUSION:TD-RAPD could be applied to researches of molecular marker of Papaver somniferum L. TD-RAPD technique provide a method to constitute DNA database of Papaver somniferum L. and conclude the source of opium poppy.
