The research of relationship between DNA degradation and postmortem interval.
- Author:
Xin CHEN
1
;
Yi-wen SHEN
;
Yun-ju GU
Author Information
1. Shanghai Institute of Criminal Science & Technology, Shanghai 200083, China. ch-sir@hotmail.com
- Publication Type:Journal Article
- MeSH:
Cell Nucleus/metabolism*;
DNA/metabolism*;
Flow Cytometry;
Forensic Medicine/methods*;
Humans;
Image Processing, Computer-Assisted/methods*;
Kidney/metabolism*;
Liver/metabolism*;
Male;
Middle Aged;
Myocardium/metabolism*;
Postmortem Changes;
Spleen/metabolism*;
Staining and Labeling/methods*;
Time Factors
- From:
Journal of Forensic Medicine
2005;21(2):115-117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the relationship between the DNA degradation in cells and postmortem interval.
METHODS:Tissue pieces of the heart, liver, spleen and kidney of one corpse (male, 54 years old, died of mechanical injury, PMI of 6h) were obtained in 6, 12, 24, 48 h after death, fixed in Carnoy fluid, and then paraffin sections were prepared, stained with Feulgen and analyzed by Image analysis technology (IAT). Meanwhile the single-cell suspension of tissues of the man was prepared and inspected by FCM after PI stained.
RESULTS:The amount of DNA of in heart, liver and kidney of human had a repid degraded in first 6 hours after death, that in the spleen showed a better relationship between DNA degradation and PMI. The results was verified by FCM and IAT.
CONCLUSION:The degradation of DNA of and human tissues shows a well relationship with PMI, especially in spleen. It is useful in estimation of PMI.
