Analysis of Gene Recombination between HLA-B and -DRB1, HLA-DQB1 and -DPB1 Loci.
10.19746/j.cnki.issn.1009-2137.2023.03.035
- Author:
Chen CHEN
1
;
Wei WANG
2
;
Nan-Ying CHEN
2
;
Li-Na DONG
2
;
Wei ZHANG
2
;
Fa-Ming ZHU
3
Author Information
1. School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang Province, China; Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China.
2. Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China.
3. Blood Center of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China; Key Laboratory of Blood Safety Research of Zhejiang Province, Hangzhou 310052, Zhejiang Province, China.E-mail: zfm00@hotmail.com.
- Publication Type:Journal Article
- Keywords:
gene recombination;
human leukocyte antigen (HLA);
next-generation sequencing
- MeSH:
Humans;
Gene Frequency;
HLA-DQ beta-Chains/genetics*;
HLA-B Antigens/genetics*;
Histocompatibility Antigens Class I/genetics*;
Haplotypes;
HLA-A Antigens/genetics*;
HLA-DRB1 Chains/genetics*;
Recombination, Genetic;
Alleles
- From:
Journal of Experimental Hematology
2023;31(3):855-859
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the recombinations within the human leukocyte antigen (HLA) region in two families.
METHODS:Genomic DNA was extracted from the peripheral blood specimens of the different family members. HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 loci were genotyped using polymerase chain reaction-sequence specific oligonucleotide probing technique (PCR-SSO) and next-generation sequencing technique. HLA haplotype was determined by genetic analysis of the pedigree.
RESULTS:The haplotypes of HLA-A*11:01~C*03:04~B*13:01~DRB1*12:02~DQB1*03:01~DPB1*05:01:01G and HLA-A*03:01~C*04:01~B*35:03~DRB1*12:01~DQB1*03:01~DPB1*04:01:01G in the family 1 were recombined between HLA-B and HLA-DRB1 loci, which formed the haplotype of HLA-A*11:01~C*03:04~B*13:01~DRB1* 12:01~DQB1*03:01~DPB1*04:01:01G. The haplotypes of HLA-A *02:06~C*03:03~B*35:01~DRB1*08:02~DQB1*04:02~ DPB1*13:01:01G and HLA-A *11:01~C*07:02~B*38:02~DRB1*15:02~DQB1*05:01~DPB1*05:01:01G in the family 2 were recombined between HLA-DQB1 and HLA-DPB1 loci, which formed the haplotype of HLA-A*02:06~C*03:03~B*35:01~ DRB1*08:02~DQB1*04:02~DPB1*05:01:01G.
CONCLUSION:The gene recombination events between HLA-B and -DRB1, HLA-DQB1 and -DPB1 loci were found respectively in two Chinese Han families.
