Content determination of seven active components of Eucommiae Cortex in aortic vascular endothelial cells of spontaneously hypertensive rats by UPLC-MS/MS.
10.19540/j.cnki.cjcmm.20230224.202
- Author:
Meng-Ting LI
1
;
He-Jia HU
1
;
Yang JIN
1
;
Yi CHEN
1
;
Si-Ying CHEN
1
;
Yue-Ting LI
1
;
Yong HUANG
1
;
Lin ZHENG
1
;
Jing HUANG
1
;
Zi-Peng GONG
1
Author Information
1. Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University Guiyang 550004, China.
- Publication Type:Journal Article
- Keywords:
(+)-pinoresinol di-O-β-D-glucopyranosid;
Eucommiae Cortex extract;
cellular pharmacokinetics;
genipinic acid;
vascular endothelial cells
- MeSH:
Rats;
Animals;
Rats, Inbred SHR;
Chromatography, Liquid;
Chromatography, High Pressure Liquid/methods*;
Endothelial Cells;
Tandem Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2023;48(13):3623-3632
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, the contents of seven active components [genipinic acid(GA), protocatechuic acid(PCA), neochlorogenic acid(NCA), chlorogenic acid(CA), cryptochlorogenic acid(CCA),(+)-pinoresinol di-O-β-D-glucopyranosid(PDG), and(+)-pinoresinol 4'-O-β-D-glucopyranoside(PG)] of Eucommiae Cortex in aortic vascular endothelial cells of spontaneously hypertensive rats(SHR) were simultaneously determined by ultra-high liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS). The qualified SHR models were selected. The primary aortic endothelial cells(VECs) of rats were separated and cultured by ligation and adherence, followed by subculture. After successful identification, an UPLC-MS/MS method for simultaneously determining the contents of GA, PCA, NCA, CA, CCA, PDG, PG in seven components of Eucommiae Cortex in VECs was established, including specificity, linearity, matrix effect, recovery, accuracy, precision and stability. The established method had the lo-west limit of quantification of 0.97-4.95 μg·L~(-1), accuracy of 87.26%-109.6%, extraction recovery of 89.23%-105.3%, matrix effect of 85.86%-106.2%, and stability of 86.00%-112.5%. Therefore, the established accurate UPLC-MS/MS method could rapidly and simultaneously determine the contents of the seven active components of Eucommiae Cortex in VECs of SHRs, which provided a refe-rence for the study of cellular pharmacokinetics of active components of Eucommiae Cortex extract.
